Toll样受体4、NF-κB在溃疡性结肠炎中的表达  

The expression of toll-like receptor4 and NF-κB in ulcerative colitis

作　　者：于振海[1] 陈立东[1] 王志强[1] 徐宁[2] 

机构地区：[1]滨州医学院人体解剖学教研室,山东烟台264003 [2]青岛大学附属烟台毓璜顶医院,山东烟台264000

出　　处：《细胞与分子免疫学杂志》2010年第7期650-652,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省自然科学基金资助项目(ZR2009CL006)

摘　　要：目的：研究Toll样受体4、NF—κB在溃疡性结肠炎（uc）中的表达变化，探讨两者在UC发病机制中的作用。方法：收集UC病例及非UC对照结肠镜活检标本或手术标本。采用免疫组化和RT—PCR技术，检测非UC对照及UC患者肠黏膜TLR4、NF—κBp65的表达水平，并做统计学分析。结果：RT—PCR结果显示，TIJR4、NF—κB065在UC组织中的表达情况显著高于在非UC对照组织的表达（TLR4：143．658±33．870，30．531±8．442，t=24．253，P〈0．01；NF—κBp65：185．773±37．625，23．810±7．038，t=31．664，P〈0．01）。免疫组化染色显示TLR4、NF—κB在UC组结肠黏膜表达增强。结论：TLR4、NF-κBp65在UC中表达高度上调，推测它们可能参与了UC的发病过程。AIM： To investigate the expression of TLR4 and NF-κB in ulcerative colitis （UC）. METHODS： Colonic biopsy specimens were collected from active UC and controis. The expression of TLR4 and NF-κBp 65 were analyzed by immunohistochemistry （IHC） and RT-PCR. RESULTS： RT-PCR revealed a significant increase of TLR4 and NF-κBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls（TLR4： 143. 658 ± 33. 870, 30. 531 ±8. 442, t = 24. 253, P 〈 0. 01 ; NF-KBp65 ： 185. 773 ± 37. 625, 23. 810± 7. 038, t = 31. 664, P 〈0. 01）. IHC show that the expression of TLR4 and NF-KB was significantly higher in colonic mucosa of UC compared with coIonic mucosa of controls. CONCLUSION： The expression of the TLR4 and NF-κB was increased in the colonic mucosa of UC compared with colonic mucosa of controls. It may be closely associated with the course of ulcerative colitis.

关 键 词：TOLL样受体4 NF-ΚBP65 溃疡性结肠炎 RT-PCR 免疫组织化学染色 

分 类 号：R392.11[医药卫生—免疫学]