基因AtHsp17.6-C2启动子的克隆及其热诱导动力学研究(英文)  

Clonning the Promoter of AtHsp17.6-C2 and Its Heat Inducible Kinetics

作　　者：曾卫军[1,2] 王水平[1] 李小方[1] 徐萍[3] 王瑞刚[4] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]新疆师范大学生命科学与化学学院,乌鲁木齐830054 [3]中国科学院上海生命科学信息中心,上海200031 [4]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《中国生物工程杂志》2010年第6期89-95,共7页China Biotechnology

基　　金：supported by The Project-sponsored by SRF for ROCS,SEM.(2006);grants from Natural Scientific Foundation of China(30660014);Natural Scientific Foundation of Inner Mongolia(200508010503)~~

摘　　要：介绍一个植物表达系统,由热激蛋白基因(AtHsp17.6-C2)的启动子来驱动GUS基因的表达。在22℃生长条件下,稳定遗传的转基因植株中几乎检测不到GUS的活性。但是当温度升至34～37℃时,GUS的活性迅速升高。37℃是该植物表达系统最适诱导温度。转基因植株经37℃热诱导2小时后再返回22℃培育2小时,GUS的活性增加80多倍。多次热诱导实验表明这个表达系统是能够被重复多次热诱导的。实验结果表明这个植物诱导表达系统能够适用于多种目的需要。A gene expression system were constructed,driven by a heat-shock gene promoter（AtHsp17.6-C2）,to control the expression of reporter gene（GUS）in transgenic plants.The expression of the AtHsp17.6-C2 chimeric gene in the stable transformants of ⊿F1-22-13 was hardly detected in culture at 22℃.However,the expression increased dramatically at the transcriptional level when the incubation temperature was shifted to 34~37℃.The optimal temperature for heat（shock）induction was 37℃.After a 2h incubation at 37℃ and 2h recovery phase at 22℃,GUS activity was about 80 fold greater than that before heat shock.Multiple heat-shock treatments showed that this system was heat reinducible.This new system can be applied in numerous and various applications.

关 键 词：拟南芥 可控基因表达 热激 AtHsp17.6-C2启动子 GUS 

分 类 号：Q786[生物学—分子生物学]