补料发酵法批量培养磁敏感氧化亚铁硫杆菌  

Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation

作　　者：刘新星[1] 郭宁[1] 梁万洁[1] 张剑[1] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083

出　　处：《中国生物工程杂志》2010年第6期109-112,共4页China Biotechnology

基　　金：国家"973"计划(2004CB619201);国家自然科学基金(50774102)资助项目

摘　　要：磁敏感氧化亚铁硫杆菌胞内可以生成磁性颗粒,探索其培养条件对趋磁细菌的研究有重要意义。利用补料发酵法,实现了磁敏感氧化亚铁硫杆菌的实验室批量培养。发酵终体积为2L的摇瓶发酵条件下,培养40h后一次补Fe2+(5%),使最高菌体浓度达到2.33×107个/ml,比不补料对照提高了60.69%;同样条件下,补入9K全料则可达到2.47×107个/ml,比不补料对照提高70.34%;原子力显微镜磁扫描结果显示发酵得到的菌体有明显磁性,即胞内含有大量磁性颗粒。Acidithiobacillus ferrooxidans（A.f）could synthesize intra-cellular nanometer magnetic particles.With the development of study on their culture conditions,the research of magnetotaxis bacteria will get more benefit from it.By fed-batch fermentation magnetic A.f could be batch cultured in the laboratory.In the shake-flask fermentation,the final volume of liquid was 2 L.After 40 h in the fermentation Fe2＋（5%）was added in the fermentation liquid.The max cell density could reach 2.33×107cells/ml.Compared with control experiments,it increased by 60.69%.At the same condition,the completion 9K culture medium was added in the fermentation liquid.The max cell density could reach 2.47×107cells/ml.Compared with control experiments,it increased by 70.34%.And atom force microscopy and magnetic force microscopy showed that cells in the fermentation were magnetic.

关 键 词：磁敏感氧化亚铁硫杆菌 补料发酵 原子力显微镜 

分 类 号：Q93-335[生物学—微生物学]