Identification of gamyb-4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development  

作　　者：Zhenhua Liu Wenjie Bao Wanqi Liang Jingyuan Yin Dabing Zhang 

机构地区：[1]School of Life Sciences, Shanghai University, Shanghai 200240, China [2]School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Journal of Integrative Plant Biology》2010年第7期670-678,共9页植物学报（英文版）

基　　金：supported by the State Key Basic Research and Development Plan of China (2009CB941500 and 2007CB108700);the High-Tech Research and Development Program of China(2006AA10A102);the National Natural Science Foundation of China (30725022 and 30600347);Shanghai Leading Academic Discipline Project (B205)

摘　　要：In higher plants, male reproductive development is a complex biological process that includes cell division and differentiation, cell to cell communication etc., while the mechanism underlying plant male reproductive development remains less understood. GAMYB encodes a gibberellins acid （GA） inducible transcription factor that is required for the early anther development in rice （Oryza sativa L.）. Here, we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon （-I-2308）, causing a frame shift and premature translational termination. Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis. To understand the regulatory role of GAMYB, we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data. These results revealed that the expression of TDR （TAPETUM DEGENERATION RETARDATION）, a tapetal cell death regulator, was downregulated in gamyb-4 and udtl （undeveloped tapetuml）. While the GAMYB expression was not obviously changed in tdr and udtl-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development. This work is helpful in understanding the regulatory network in rice anther development.In higher plants, male reproductive development is a complex biological process that includes cell division and differentiation, cell to cell communication etc., while the mechanism underlying plant male reproductive development remains less understood. GAMYB encodes a gibberellins acid （GA） inducible transcription factor that is required for the early anther development in rice （Oryza sativa L.）. Here, we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon （-I-2308）, causing a frame shift and premature translational termination. Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis. To understand the regulatory role of GAMYB, we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data. These results revealed that the expression of TDR （TAPETUM DEGENERATION RETARDATION）, a tapetal cell death regulator, was downregulated in gamyb-4 and udtl （undeveloped tapetuml）. While the GAMYB expression was not obviously changed in tdr and udtl-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development. This work is helpful in understanding the regulatory network in rice anther development.

分 类 号：Q78[生物学—分子生物学] S512.1[农业科学—作物学]