人白细胞介素-10的克隆和表达  

The Cloning and Expression of Human Interleukin 10

作　　者：牛亚梅[1] 王旻[1] 胡容[1] 张玉彬[1] 

机构地区：[1]中国药科大学生物制药学院

出　　处：《药物生物技术》1999年第1期5-8,共4页Pharmaceutical Biotechnology

摘　　要：构建了含有人白细胞介素１０基因的重组质粒并在大肠杆菌中进行非分泌表达。表达蛋白经还原性聚丙烯酰胺凝胶电泳鉴定Ｍｒ１８０００，经酶联免疫测定具有免疫活性，薄层扫描测知表达效率约２１．６％。本实验为进一步研制人白介素１０的基因工程药物奠定了基础。The recombinant bector vector including hIL 10 was constructed and expressed in BL 21(DE 30). The M r of hIL 10 was estimated to be 18 000 by SDS PAGE.It was proved to be immunological active by the result of ELISA.The expression efficiency was 21.6% by thin screeing.This report laid a foundation for exploting the medical value of hIL 10 as a genetic engineering drug.

关 键 词：人白细胞介素10 克隆 表达 

分 类 号：R392－33[医药卫生—免疫学] Q78[医药卫生—基础医学]