日本血吸虫不同发育阶段形态与生长速度差异分析  

Analyzing differences in growth rates of Schistosoma japonicum worms at various points in development

作　　者：方会龙[1,2] 曾庆仁[1] 王俊杰[2] 曾铁兵[1,3] 喻容[1] 杨胜辉[1] 刘碧源[1,3] 刘彦[1,3] 蔡力汀[1] 

机构地区：[1]中南大学医学细胞与分子生物学,湖南长沙410013 [2]湘南学院基础医学课部,湖南郴州423000 [3]南华大学医学院病原生物学研究所,湖南衡阳421001

出　　处：《中国病原生物学杂志》2010年第6期437-439,F0003,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金资助项目(No.30570952);湖南省自然科学基金资助项目(No.07JJ3031);湖南省教育厅课题资助项目(No.08C819)

摘　　要：目的观察日本血吸虫不同发育阶段的生长速度差异。方法采用清洁级昆明鼠,经人工感染日本血吸虫尾蚴,分别收集感染前0 d和感染后第8、12、16、20、24和28 d的虫体,用Motic BA400病理图像处理系统及3.2分析软件采集虫体图象,作形态观察和多指标测量。结果发育中的童虫在不同阶段生长速度不均衡,以8-12 d时生长最快,其他时段依次为12-16 d、16-20 d、20-24 d、0-8 d和24-28 d;同一阶段的虫体发育差异较大,尤其在12 d后。结论日本血吸虫童虫在不同发育时段的生长速度存在明显差异;8~12 d时虫体生长最快。Objective To observe the differences in growth rates of Schistosoma japonicum（Sj）at different points in development.Methods Worms were recovered from Kunming mice infected with Sj cercariae on days 0,8,12,16,20,24,and 28 post-infection,respectively.Pathological Image Processing System Motic BA400 and 3.2 software were utilized to observe morphology and measure worm area,perimeter,length,and width.Results Worms at different points in development grew at distinctive rates.Worms on days 8 to 12 had the fastest development,followed by worms on days 12 to 24,0 to 8,and 24 to 28.Even at the same point in time,there were obvious distinctions among the worms,especially after day 12.Conclusion The growth rate of Sj worms differs at different points in the worms＇ development.The growth rate of 8 to 12-day old worms was the fastest.

关 键 词：血吸虫 日本 发育阶段 形态 生长速度 

分 类 号：R383.24[医药卫生—医学寄生虫学]