Effects of Bushen Tongmai Recipe(补肾通脉方) on Protein Kinase Bα Expression in Polycystic Ovary Rats with Insulin Resistance  

作　　者：李琼 黄冬梅 陆付耳 谢阳 徐丽君 邹欣 龚菂 王增四 

机构地区：[1]Institute of Integrated Traditional and Western Medicine,Affiliated to Tongji Hospital,Tongji Medical College,Huazhong University of Sciences and Technology

出　　处：《Chinese Journal of Integrative Medicine》2010年第4期324-330,共7页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.30600838)

摘　　要：Objective： To observe the effects of Bushen Tongmai Recipe （补肾通脉方, BSTMR） on mRNA and protein expressions of protein kinase B alpha （PKB α） in hepatic, adipose, muscular, and ovarian tissues of polycystic ovary（PCO） rats with insulin resistance （IR） and to explore the possible molecular mechanism of BSTMR in treating IR and ovulation dysfunction. Methods： Female 22-day-old SD rats were injected subcutaneously with sodium prasterone sulfate （9 mg.100g^-1.d^-1） for 20 days and fed with high-fat diet for 80 days to induce PCO rats with IR. Then, the PCO rats were randomly divided into the model group （n=23） and the treated group （n=21）. The treated group was administered with BSTMR for 2 weeks. Meanwhile, a group with 15 rats of the same age was used as the control group. The histological changes in the ovaries were examined. Fasting blood glucose （FBG） was determined by the glucose oxidase method. Serum fasting insulin （Fins） was determined by radioimmunoassay （RIA）. The mRNA level of PKB ~ was measured by reverse transcription polymerase chain reaction （RT-PCR）. Immunohistochemistry staining and Western blot analysis were employed to detect the protein expression in target tissues. Results： Compared with the control group, the ovaries in the model group showed multiple follicular cysts, levels of FBG and Fins in the model group increased markedly （P〈0.05 or P〈0.01, respectively）, and the insulin sensitive index （ISI） decreased obviously （P〈0.01）. The mRNA and protein expressions of PKB e in target tissues in the model group were dramatically lower than those in the control group （P〈0.05 or P〈0.01）. Compared with the model group, the stratum granulosum of the ovarian follicle in the treated group increased markedly, the level of Fins in the treated group decreased obviously （P〈0.01）, ISI in the treated group improved markedly （P〈0.01）, and the mRNA and protein expressions of PKBα in target tissues of the treated ratObjective： To observe the effects of Bushen Tongmai Recipe （补肾通脉方, BSTMR） on mRNA and protein expressions of protein kinase B alpha （PKB α） in hepatic, adipose, muscular, and ovarian tissues of polycystic ovary（PCO） rats with insulin resistance （IR） and to explore the possible molecular mechanism of BSTMR in treating IR and ovulation dysfunction. Methods： Female 22-day-old SD rats were injected subcutaneously with sodium prasterone sulfate （9 mg.100g^-1.d^-1） for 20 days and fed with high-fat diet for 80 days to induce PCO rats with IR. Then, the PCO rats were randomly divided into the model group （n=23） and the treated group （n=21）. The treated group was administered with BSTMR for 2 weeks. Meanwhile, a group with 15 rats of the same age was used as the control group. The histological changes in the ovaries were examined. Fasting blood glucose （FBG） was determined by the glucose oxidase method. Serum fasting insulin （Fins） was determined by radioimmunoassay （RIA）. The mRNA level of PKB ~ was measured by reverse transcription polymerase chain reaction （RT-PCR）. Immunohistochemistry staining and Western blot analysis were employed to detect the protein expression in target tissues. Results： Compared with the control group, the ovaries in the model group showed multiple follicular cysts, levels of FBG and Fins in the model group increased markedly （P〈0.05 or P〈0.01, respectively）, and the insulin sensitive index （ISI） decreased obviously （P〈0.01）. The mRNA and protein expressions of PKB e in target tissues in the model group were dramatically lower than those in the control group （P〈0.05 or P〈0.01）. Compared with the model group, the stratum granulosum of the ovarian follicle in the treated group increased markedly, the level of Fins in the treated group decreased obviously （P〈0.01）, ISI in the treated group improved markedly （P〈0.01）, and the mRNA and protein expressions of PKBα in target tissues of the treated rat

关 键 词：polycystic ovary insulin resistance Bushen Tongmai Recipe protein kinase Bα 

分 类 号：R285.5[医药卫生—中药学]