Effect of acupuncture on the luteal function of rats with embryo implantation dysfunction  

针刺对胚胎着床障碍大鼠黄体功能的影响(英文)

作　　者：何丹娟[1] 黄光英[1] 张明敏[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所

出　　处：《World Journal of Acupuncture-Moxibustion》2010年第2期32-37,共6页世界针灸杂志（英文版）

基　　金：Supportedt by the National Nature Science Foundation:90209009

摘　　要：Objective To observe the effects of acupuncture on the luteal function of rats with embryo implantation dysfunction, and to explore its mechanism. Methods The early pregnant rats were randomly divided into normal control group （N）, model group （M）, acupoint group （A）, and non-acupoint group （AC）. The model of embryo implantation dysfunction in Group M, A, and AC was established with Mifepristone. For rats in Group A, bilateral ＂Housanli＂ （后三里 ST 36） and ＂Sanyinjiao＂ （三阴交 SP 6） were needled, while the non-acupoints beside the acupoints were needled in Group AC. In this experiment, the serum levels of luteinizing hormone （LH）, estradiol （E2）, and progesterone （P） were detected with radioimmunoassay, the expression of vascular endothelial growth factor （VEGF） in the rat ovarian tissue was detected by using Western-blot. The mRNA expressions of VEGF and luteinizing hormone receptor （LHR） in the ovarian tissue were detected by using RT-PCR. Results The serum levels of LH and P were significantly higher in group A than in group M and AC （all P〈0.05）, showing no statistical significance when compared with group N. The VEGF content, and expressions of VEGF mRNA and LHR mRNA in the ovarian tissue of group A were significantly elevated than those in group M and group AC （all P〈0.05）, showing no statistical significance when compared with group N. Conclusion Needling at ＂Housanli＂ （后三里 ST 36） and ＂Sanyinjiao＂ （三阴交 SP 6） could elevate the serum levels of LH and P of rats with embryo implantation dysfunction, and up-regulate the expression of LHR mRNA, VEGF and its mRNA in the ovarian tissue. It may enhance the luteal function of rats with embryo implantation dysfunction and improve its embryo implantation environment.目的:观察针刺对胚胎着床障碍大鼠黄体功能的影响,探讨其作用机制。方法:将早孕大鼠随机分为正常组(N), 模型组(M), 针刺穴位组(A), 针刺非穴位组(AC),M组, A组, AC组均采用米非司酮造模。A组针刺大鼠双侧"后三里""三阴交",AC组针刺其穴位旁开非穴位点。放免法检测各组大鼠血清黄体生成素(LH), 雌二醇(E2), 孕酮(P)的水平;免疫组化, Western-blot方法检测各组大鼠卵巢组织血管内皮生长因子(VEGF)的表达;RT-PCR方法检测各组卵巢组织黄体生成素受体(LHR)mRNA及VEGF mRNA的表达。结果:A组血清LH, P水平显著高于M组, AC组(均P<0.05),与N组比较差异无统计学意义;A组卵巢组织VEGF含量, LHR mRNA, VEGF mRNA表达水平较M组, AC组显著提高(均P<0.05),与N组比较差异无统计学意义。结论:针刺"后三里""三阴交"可升高胚胎着床障碍大鼠血清P, LH水平,上调卵巢组织LHRmRNA, VEGF及其mRNA表达水平,可在一定程度上增强大鼠黄体功能,改善胚胎着床环境。

关 键 词：Acupuncture Therapy RECEPTORS LH PROGESTIN Vascular Endothelial Growth Factors 

分 类 号：R245[医药卫生—针灸推拿学]