日本牡丹品种花粉超低温保存  

Pollen cryopreservation of Japanese tree peony cultivars

作　　者：李秉玲[1] 王荷[2] 刘燕[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术研究中心 [2]苏州农业职业技术学院园林与园艺系

出　　处：《北京林业大学学报》2010年第4期297-300,共4页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30972411)

摘　　要：为解决杂交育种工作中的时空不遇,延长花粉寿命,本文对23个日本牡丹品种花粉进行了超低温保存研究。对新鲜花粉及超低温保存花粉离体萌发率测定结果表明:不同日本牡丹品种新鲜花粉生活力存在差异,23个品种中,‘大藤锦’花粉萌发率最高,达到60.89%,而‘岛大臣’最低,仅3.49%;室温条件下,日本牡丹品种花粉寿命仅15d左右,而超低温保存可以使日本牡丹品种花粉寿命至少延长至2年。经超低温保存2年,花粉仍然具有较高的萌发率,有的甚至超过保存前水平,因此超低温保存技术可用于日本牡丹品种花粉的长期保存。In order to prolong pollen viability,pollen of 23 Japanese tree peony （ Paeonia suffruticosa Andr.） cultivars were cryopreserved in liquid nitrogen.In vitro germination of both fresh and cryopreserved pollen was examined by the hanging-drop method.The results showed that the germination rate of fresh pollen varied with cultivars,with the highest value （60.89% ） in P.‘Shimane Chojuraku’and the smallest （3.49% ） in P.‘Shimadaijin’.At room temperature,pollen viability of most cultivars was lost within 15 days,but cryopreservation prolonged pollen viability for at least two years.After two years,cryopreserved pollen still retained a high germination rate,which was even higher than that of fresh pollen in some cultivars.We conclude that cryopreservation is applicable for long-term preservation of pollen of Japanese tree peony.

关 键 词：日本牡丹 花粉 萌发率 超低温保存 

分 类 号：S685.11[农业科学—观赏园艺]