经穴效应特异性在穴位注射多巴酚丁胺增效作用中的体现  

A Reinforcement of Acupuncture on the Cardiac Effect of Dobatamine:Exhibition of Acupoint's Functional Specificity

作　　者：崔晶晶[1] 高俊虹[2] 付卫星[2] 王玉敏[1] 马淑骅[1] 张萌[3] 崔海峰[1] 喻晓春[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院针灸研究所,北京100700 [3]辽宁医学院药理教研室,锦州121001

出　　处：《针刺研究》2010年第3期188-192,共5页Acupuncture Research

基　　金：北京市自然科学基金(7072053);国家自然科学基金(30873295);科技部"973"计划专项课题(2006CB504506)

摘　　要：目的:探讨药物穴位注射是否体现穴位效应的相对特异性。方法:SD大鼠随机分为正常对照组、电针内关+穴位注射组(EA-PC6+AI组)、电针足三里+穴位注射组(EA-ST36+AI组)和电针常规肌肉注射部位+注射组(EA-IMIS+IMI组),每组8只。电针各组分别电针10min后,再于相应部位注射β1-肾上腺素受体的激动剂盐酸多巴酚丁胺(DH,100μg/kg)。分析比较相应部位电针+注射DH前,电针+注射DH后2、5、15和30min左心室收缩压(LVSP)、心率(HR)和左心室最大上升速率(+dp/dtmax)变化。结果:相应部位电针+注射DH后2min时,以EA-PC6+AI组增加LVSP、HR和+dp/dtmax的药理作用表现最为明显(各组相应指标与同时间点EA-PC6+AI组比较P<0.01或P<0.05)。注射DH后30min时,EA-ST36+AI和EA-IMIS+IMI组心功能各项指标均有较大幅度的回落,而EA-PC6+AI组LVSP、HR和+dp/dtmax的回落幅度最低(与同时间点正常对照组比较均P<0.01)。结论:电针"内关"穴加穴位注射确实有作用较强、作用时间窗宽大等优势,体现了其对心脏功能影响的相对特异性。Objective To investigate the functional specificity of acupoint by means of acupoint injection of Dobutamine Hydrochloride （DH）. Methods Male SD rats were randomized into normal control （NC）, EA-Neiguan （PC6） ＋ acupoint injection （AI, EA-PC 6 ＋ AI）, EA-Zusanli （ST 36） ＋ Al（EA-ST 36 ＋ AI）, and EA-intramuscular injection site （IMIS） ＋ intramuscular injection （EA-IMIS＋ IMI） groups,with 8 rats being in each group. EA （2 Hz/15 Hz, 3 mA） was applied to bilateral ＂Neiguan＂ （PC 6）, ＂Zusanli ＂（ST 36） and muscular-injection site for 10 min. DH （a β1-adrenoceptor agonist） solution （100 μg/kg） was injected intramuscularly into the gluteus maximus after termination of EA intervention. The left ventricular systolic pressure （LVSP）, heart rate （HR） and ＋ dp/dt max were recorded before and 2, 5, 15 and 30 min after injection of DH. Results The values of LVSP, HR and ＋ dp/dt max in EA-PO 6 ＋ AI group were significantly higher than those in NC, EA-ST 36 ＋ AI, and EA-IMIS ＋ IMI groups （P〈0.01） 2 and 5 minutes after EA plus acupoint injection of DH. No significant differences were found between EA-ST 36 ＋ AI and EA-IMIS＋ IMI groups in LVSP, HR and ＋ dp/dt max （P〉0.05）. After injection of DH for 30 minutes, the values of LVSP, HR and ＋ dp/dt max in EA-PC 6 ＋ AI group were still significantly higher than those in NC group （P〈0.01）. Conclusion EA-PC 6 ＋ AI DH is significantly superior to EA-ST 36＋ AI DH and EA-IMIS＋ IMI DH in producing a stronger cardiac excitatory effect and a longer post-effect, showing a relative specificity of the acupoint in upregulating cardiac functional activities.

关 键 词：内关穴 电针 穴位注射 经穴效应特异性 心脏效应 

分 类 号：R245.95[医药卫生—针灸推拿学]