HPAA在艾灸调控类风湿性关节炎大鼠滑膜组织NF-kB信号通路中的作用  

Involvement of the Hypothalamus-pituitary-adrenal Axis in Moxibustion-induced Changes of NF-kB Signaling in the Synovial Tissue in Rheumatic Arthritis Rats

作　　者：高骏[1] 刘旭光[1] 黄迪君[1] 唐勇[1] 周海燕[1] 尹海燕[1] 陈婷[1] 

机构地区：[1]成都中医药大学针灸推拿学院,成都610075

出　　处：《针刺研究》2010年第3期198-203,共6页Acupuncture Research

基　　金：国家自然科学基金(No.30672722);教育部新世纪优秀人才计划资助

摘　　要：目的:通过对下丘脑-垂体-肾上腺轴(HPAA)在艾灸调控类风湿性关节炎(RA)大鼠滑膜组织核转录因子(NF-kB)信号通路中的作用分析,深入挖掘艾灸治疗RA抗炎效应的细胞信号转导作用机制。方法:雄性SD大鼠50只,随机分为空白组、模型组、艾灸组、摘肾上腺组、假手术组,每组各10只。右后足足垫部皮内注入福氏完全佐剂0.1mL制作RA模型。造模成功后第6天手术摘除摘肾上腺组大鼠双侧肾上腺。造模第7天开始艾灸大鼠"肾俞""足三里"两穴各5壮,每日1次,治疗18d。观测计算大鼠足跖肿胀度,采用ELISA双抗夹心法检测大鼠血浆促肾上腺皮质激素(ACTH)和血清皮质醇(CS)含量,运用免疫组化法测定大鼠滑膜NF-kBp65活性。结果:造模后大鼠右后足容积,模型组、艾灸组、摘肾上腺组和假手术组显著大于空白组(P<0.01);艾灸后大鼠右后足容积,艾灸组、假手术组显著小于模型组(P<0.01),摘肾上腺组显著大于空白组、艾灸组(P<0.01,P<0.05)。大鼠夜间血浆ACTH水平,模型组显著低于空白组(P<0.05),艾灸组、假手术组显著高于模型组(P<0.05),摘肾上腺组显著低于空白组、模型组、艾灸组(P<0.01)。大鼠夜间血清CS水平、滑膜NF-kBp65活性,模型组显著高于空白组(P<0.01),艾灸组、假手术组显著低于模型组(P<0.01),摘肾上腺组CS水平显著低于空白组、模型组、艾灸组(P<0.05),而滑膜NF-kBp65活性显著高于空白组、艾灸组(P<0.01)。结论:艾灸治疗实验性RA大鼠具有确切的抗炎效应,其抗炎效应的发挥需依赖于HPAA的完整。艾灸可能通过调节HPAA功能,从而抑制NF-kB炎性信号通路而发挥抗炎作用。Objective To observe the effect of moxibustion of ＂Shenshu＂ （BL 23） and ＂Zusanli＂（ST 36） on synovial nuclear factor （NF）-kB p 65 expression, and plasma adrenocorticotropic hormone （ACTH） and serum cortisol （CS） contents in rheu- matic arthritis （RA） rats with adrenalectomy （ADX） so as to study the underlying mechanism in ameliorating RA. Methods Fifty male SD rats were randomly divided into control （n = 10）, model （n = 10）, moxibustion （n = 10）, ADX （n =10） and false ADX （n=10） groups according to SPSS-aided random digits table. RA model was established by injecting 0. 1 mL Freund＇s complete adjuvant into the right paw. ADX operation was performed on the sixth day after successful establishment of RA model. Moxibustion was given to ＂Shenshu＂（BL 23） and ＂Zusanli＂（ST 36） from the 7th day on, five moxa cones each session, and once daily for 18 days. Then plasma ACTH and serum CS contents, for which the blood was collected during night, were detected by ELISA, and NF-kB p 65 immunoactivity in synovial tissue was detected by immunohistochemistry. Results In comparison with control group, the foot volumes （swelling degree） of model, moxibustion, ADX, and false ADX groups increased significantly （P〈0.01）, while compared with model group, the swelling degrees of moxibustion and false ADX groups were decreased remarkably after the treatment （P〈0.01）. No significant difference was found between ADX and model groups in the foot volume （P〉0.05）, Compared with control group, plasma ACTH content in RA model group decreased obviously （P〈0.05）, while serum CS level and NF-kB p 65 immunoactivity increased apparently in model group （P〈0. 01）. Compared with model group, serum CS contents in moxibustion, ADX and false ADX groups and synovial NF-kB p 65 immunoactivity in moxibustion and false ADX groups reduced considerably （P〈0.01, P〈0.05）. No significant difference was found between ADX and model groups in synovial NF

关 键 词：艾灸 类风湿性关节炎 HPAA 滑膜NF-kB P65 

分 类 号：R245.81[医药卫生—针灸推拿学]