高效液相色谱分析红细胞膜磷脂各组分  

Separation and Quantification of Erythrocyte Membrane Phospholipid Classes by High Performance Liquid Chromatography

作　　者：马焕文[1] 郭述苏[2] 于盛茂[3] 

机构地区：[1]济南军区总医院血液科,250031 [2]济南军区总医院神经内科,250031 [3]济南军区药检所

出　　处：《中国微循环》1999年第1期55-56,共2页Journal of Chinese Microcirculation

摘　　要：目的探讨红细胞膜磷脂组分的高效液相测定方法。方法选用 Sｐｈｅｒｉｓｏｒｂ硅胶色谱柱，流动相为乙腈－甲醇－８５％磷酸（１００：１０：１.８Ｖ／Ｖ／Ｖ），波长２０３ｎｍ。结果４分钟内完全分离膜的四种磷脂组分磷脂酰丝氨酸（ＰＳ）、磷脂酰乙醇胺（ＰＥ）、磷脂酰胆碱（ＰＣ）和神经鞘磷脂（ＳＭ），最低检出限为１μｇ／ｍｌ，至少在４μｇ－２０００μｇ／ｍｌ范围内标准品浓度与峰面积积分值呈线性关系，ＰＳ、ＰＥ、ＰＣ、ＳＭ的回收率分别为９８.１±２.３％、９９．８±０.７％、９９.４±１.８％、９０．６±２．２％。结论本法适于临床检测和科研探索。Objective To study the method for the quantification of human erythrocyte membrane phospholipid classes by high performance liquid chromatography. Methods The separation was performed on Spherisorb SI silica gel columns with a mobile phase of acetonitrile-methanol -85% phosphoric acid (100: 10: 1. 8 V/V/V) and UV detection at 203nm. Results Complete separation of phosphatidylserine (PS), phosphatidylethanolamin (PE), phosphatidylcholine (PC) and sphingomyelin (SM) was achieved within 4 minutes. The detection limit is 1μg/ml. The phospholipid concentrations and the peak-area values exhibited a linear relationship at least in the range 4-2000μg/ml.The recoveries of PS,PE,PC and SM were 98.1±2.3%, 99.8±0.7%, 99.4±1.8% and 90.6±2.2% respectively. Conclusion This method is suitable for clinical determination and scientific research.

关 键 词：高效液相色谱 红细胞膜 磷脂 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] Q545.03[医药卫生—基础医学]