HPV的负荷量、hTERC扩增在子宫颈癌变中的意义  

Significance of HPV DNA load and hTERC gene amplification in cervical carcinogenesis

作　　者：齐丽敏[1] 周颖[1] 朱园园[1] 刘巧[1] 卫莹[1] 李敏[1] 冯定庆[1] 李彩荣[1] 凌斌[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科分子实验室,安徽合肥230001

出　　处：《中国妇幼保健》2010年第19期2720-2723,共4页Maternal and Child Health Care of China

基　　金：安徽省自然科学基金项目〔090413117〕

摘　　要：目的:研究不同程度子宫颈病变中HPV负荷量和端粒酶(human telomerase gene,hTERC)基因的扩增情况,以探讨两者在宫颈癌发生发展中的作用及相关性。方法:采用第二代杂交捕获(HCⅡ)技术检测宫颈脱落细胞HPV-DNA含量并用荧光原位杂交(fluorescent in situ hybridization,FISH)方法对HCⅡ检测剩余宫颈细胞保存液进行hTERC检测。结果:①随着宫颈病变严重程度的增加,高危型HPV的病毒负荷量也增高,慢性宫颈炎组与CINⅠ组、CINⅡ、CINⅢ和宫颈癌组比较,差异有统计学意义(P<0.05);CINⅠ组与CINⅡ、CINⅢ和宫颈癌组比较差异有统计学意义(P<0.05);CINⅡ组、CINⅢ组和宫颈癌组之间比较差异无统计学意义(P>0.05)。②随着宫颈病变程度的加深,hTERC基因扩增阳性率逐渐升高。hTERC基因扩增阳性率除慢性宫颈炎组、其他各组与正常组对比均有统计学意义差异(P<0.05);慢性宫颈炎组与CINⅠ组比较无统计学意义差异(P>0.05),与CINⅡ、CINⅢ和宫颈癌组比较有统计学意义差异(P<0.05);CINⅠ组、CINⅡ组、CINⅢ组与宫颈癌组比较差异有统计学意义(P<0.05)。③随着HPV-DNA负荷量的增加,hTERC基因扩增阳性率也逐渐升高(r=0.995,P<0.01)。结论:HPV-DNA负荷量的增加与hTERC基因扩增在宫颈癌前病变及宫颈癌的发生发展中可能起了重要的作用,可作为子宫颈癌前病变和宫颈癌筛查的监测指标。Objective：To investigate the status of HPV load and human telomerase gene （hTERC） amplification in cervical lesions at different degrees, explore the functions of HPV load and hTERC gene amplification in occurrence and development of cervical carcinoma and correlation.Methods：HPV DNA content in cervical exfoliated cells was detected by hybrid capture Ⅱ （HC-Ⅱ）, then hTERC detection was conducted in the preservation solution of residual cervical cells by fluorescence in situ hybridization （FISH）.Results：With the deterioration of cervical lesions, the high risk HPV load increased, there was significant difference among chronic cervicitis group, CIN Ⅰ group, CIN Ⅱ group, CIN Ⅲ group and cervical carcinoma group （P0.01）.There was significant difference between CIN Ⅰ group, CIN Ⅱ group, CIN Ⅲ group and cervical carcinoma group （P0.05）; and there was no significant difference between CIN Ⅱ group, CIN Ⅲ group and cervical carcinoma group （P 0.05）.With the deterioration of cervical lesions, the positive rate of hTERC gene amplification increased gradually; there was significant difference in positive rate of hTERC gene amplification between normal group and the other groups except chronic cervicitis group （P0.05）.There was no significant difference between chronic cervicitis group and CIN Ⅰ group （P 0.05）; there was significant difference between chronic cervicitis group, CIN Ⅰ group and CIN Ⅱ group, CIN Ⅲ group, cervical carcinoma group （P0.05）; there was significant difference between CIN Ⅰ group, CIN Ⅱ group, CIN Ⅲ group and cervical carcinoma group （P0.05）; With the increase of HPV DNA load, the positive rate of hTERC gene amplification increased gradually （r=0.995,P0.01）.Conclusion：Increase of HPV DNA load and hTERC gene amplification play important role in occurrence and development of cervical carcinoma, which can be used as monitoring indicators of screening of precancerous lesion of cervix and cervical carcinoma.

关 键 词：人乳头瘤病毒 负荷量 荧光原位杂交 端粒酶基因 宫颈癌 

分 类 号：R711.74[医药卫生—妇产科学] R737.33[医药卫生—临床医学]