流产孕妇HBV-DNA含量与染色体着丝粒点变异研究  

Study on HBV-DNA content and variation of centromere of chromosome in spontaneous abortion women

作　　者：刘彦轩[1] 贾安奎[1] 

机构地区：[1]新乡医学院第一附属医院遗传研究室,河南卫辉453100

出　　处：《中国妇幼保健》2010年第19期2738-2740,共3页Maternal and Child Health Care of China

基　　金：河南省教育厅自然科学研究项目〔2009A330004〕

摘　　要：目的:通过对感染乙肝病毒的流产孕妇外周血HBV-DNA含量检测和其淋巴细胞染色体着丝粒点改变研究分析,探讨孕妇自然流产原因。方法:选择47例患有乙型肝炎自然流产史的孕妇,采用荧光定量PCR法检测HBV-DNA含量,分为103～5(Ⅰ组)和>105(Ⅱ组)两组;选取15例健康体检者作为对照组。对上述各组人员进行外周血淋巴细胞染色体制作,G显带核型分析和同步银染法观察染色体着丝粒点变异情况,分析变异率与HBV-DNA含量的关系。结果:前两组外周血淋巴细胞染色体着丝粒点变异率均高于正常对照组,Ⅰ组与对照组、Ⅰ组与Ⅱ组之间均有显著性差异,Ⅱ组与对照组有极显著性差异,病毒含量越高,变异率也越大。结论:乙型肝炎病毒可引起外周血染色体着丝粒点变异,变异率随病毒含量的升高而增加,从而导致染色体不分离,提示乙肝患者Cd的变化是导致流产的重要原因之一。Objective：To explore the causes of spontaneous abortion by detection of HBV-DNA content in peripheral blood of HBV infected pregnant women with spontaneous abortion and analyzing variation of centromere of chromosome in lymphocytes.Methods：47 HBV infected pregnant women with the history of spontaneous abortion were selected, HBV-DNA content was detected by fluorescence quantitative PCR, then the pregnant women were divided into 103～5group （Ⅰ group） and105 group （Ⅱ group）; 15 healthy women were selected as control group; chromosomes in lymphocytes of peripheral blood were prepared, G binding karyotype analysis and synchronous silver staining were used to observe the variation of centromere, the relationship between rate of variation and HBV-DNA content was analyzed.Results：The variation rates of centromere of chromosome in lymphocytes of peripheral blood in the former two groups were significantly higher than that in control group; there was significant difference among I group, II group and control group, the rate of variation increased with the increase of HBV-DNA content.Conclusion：HBV can induce the variation of centromere of chromosome in peripheral blood, the rate of variation increases with the increase of HBV-DNA content, which induces chromosome segregation and indicates that change of centromeric dot is an important cause of abortion.

关 键 词：流产 HBV-DNA 着丝粒点 

分 类 号：R714.21[医药卫生—妇产科学]