豚鼠迷路震荡造模初探  

Labyrinthine Concussion Model Exploration

作　　者：徐鸥[1] 李志玉[1] 刘砚星[2] 张燕卓[1] 路虹[1] 

机构地区：[1]河北医科大学第二医院耳鼻喉科,石家庄050000 [2]河北医科大学药理教研室

出　　处：《听力学及言语疾病杂志》2010年第4期355-358,共4页Journal of Audiology and Speech Pathology

基　　金：河北省科技支撑计划项目(08276101D-35)

摘　　要：目的借助自制单摆打击装置建立脑震荡动物模型,探讨迷路震荡动物模型的建立方法。方法将40只健康豚鼠随机分为对照组、撞击后4周组、撞击后6周组和撞击后8周组,每组10只。在清醒状态下以自制的单摆打击装置对实验组动物头部实施一次性打击,对照组不予打击。实验前后测试动物双耳DPOAE和ABR。测试结束后断头处死动物,取出耳蜗制作光镜和透射电镜标本,观察各组动物耳蜗螺旋器和螺旋神经节的病理改变;实验各组随机抽取2只动物行脑组织切片,HE染色,观察脑神经细胞形态。结果实验组动物头部受打击后无颅骨骨折,脑组织病理切片可见神经细胞肿胀和轴索断裂;与正常对照组比较,撞击后4周组DPOAE在0.5、0.7、1、2kHz幅值降低;撞击后6周组仅2kHz幅值降低,撞击后8周组仅1、2kHz幅值降低(均P<0.05);各实验组与正常对照组比较ABR反应阈降低(P<0.05)。撞击后4周组和6周组螺旋器隧道变形,毛细胞排列不整齐,撞击后8周组螺旋器及毛细胞未见明显变化;透射电镜发现实验各组螺旋神经节细胞均有不同程度受损。结论自制单摆打击装置可成功建立脑震荡动物模型;动物脑震荡后听力及耳蜗毛细胞功能及螺旋神经节病理变化符合迷路震荡特点;脑震荡动物模型可适用于制作迷路震荡动物模型。Objective To establish a labyrinthine concussion model by using self--made pendulum shocking model of cerebral concussion. Methods Forty healthy guinea pigs were randomly divided into 4 groups, 10 animals per group： normal control group; four weeks post--concussion group; six weeks post--concussion group; eight weeks post--concussion group. The disposable attack was implemented by the self made pendulum shocking device to the experimental group animals forehead under the coaking condition, while the control group did not receive the same attack. Before and after the treatment, animal binaural distortion product otoacoustic emissions （DPOAE） and auditory brainstem response （ABR） were tested. One day after the experiment test, the animals were decapitated. Otic vesicles were removed immediately, and the cochleas were fixed with different fixatives. The specimens were prepared for light microscopy （LM） and transmission electron microscopy （TEM）. Pathology change of animal cochlea spiral organ and the spiral ganglion in each group were observed. Of 2 animals in each group were randomly .scleclcd for brain tissue 6lice, hematoxylin eosin （HE） dyes, and cerebral nerve cellular morphology observation. After the experimental animals＂ forehead were attacked, skull fracture did not happen. Nerve cell swelling and the axis cylinder broken were observed on brain pathological speciments. Results The DPOAE amplitude at 0.5 kHz, 0.7 kHz. 1 kHz and 2 kHz in the four weeks post-concussion group were lower than those of normal control group （P〈0.05）, the amplitude at 2 kHz in the six weeks post concussion group was lower than that of normal control group （P〈0.05）, the amplitude at 1 kHz and 2 kHz in the eight weeks post--concussion group were lower than those of normal control group （P〈0.05）. The ABR thresholds in all experiment groups were significantly changed than that of control group （P〈0.05）. The structure of Corti＇s organ was damaged severely in four weeks post-- concussion

关 键 词：豚鼠 迷路震荡 动物模型 耳蜗 螺旋神经节 

分 类 号：R764.34[医药卫生—耳鼻咽喉科]