根癌农杆菌介导花生高效遗传转化体系的优化  

An effective Agrobacterium tumefaciens-mediated transformation system of peanut ( Arachis hypogaea L. )

作　　者：邱金梅[1,2] 温世杰[1] 刘海燕[1] 梁炫强[1] 

机构地区：[1]广东省农业科学院作物研究所,广东广州510640 [2]华南师范大学生命科学学院,广东广州510631

出　　处：《中国油料作物学报》2010年第2期208-211,共4页Chinese Journal of Oil Crop Sciences

基　　金：现代农业产业技术体系建设专项资金;粤港关键领域重点突破项目(200849863);国家自然科学基金(30971819);广东省农业科学院院长基金项目;国家高技术研究发展计划(2006AA10Z156)

摘　　要：本文研究表面活性剂(MES,methyl ester sulfonate)、真空渗入法和二次侵染对提高花生转化效率的影响,建立了一种农杆菌介导的花生快速高效遗传转化体系。该体系以带子叶的胚轴为外植体,在OD600为0.7的菌液中,加入150mg/L的MES,40kPa真空处理10min,共培养3d后进行二次侵染。采用该体系可显著提高遗传转化率,GUS基因阳性表达率最高,达73.3%。Impact of surfactant （ MES,Methyl ester sulfonate） ,vacuum infiltration and twice infection treat-ments on peanut （ Arachis hypogaea L. ） trans-efficiency was studied. The object was to construct a high efficient Agrobacterium tumefaciens-mediated transformation system. The explant of hypocotyl with cotyledons were cultured in the pMMO which value in OD600was 0. 7,adding the MES in a concentration of 150mg/L,and treated in vacuum at 40kPa for 10 minutes. The explant was infected for the second time after being cultured for 3 days. This system could apparently improve the efficiency of transformation,in which the GUS gene could be expressed as high as 73. 3% .

关 键 词：花生 根癌农杆菌介导 遗传转化 

分 类 号：Q789[生物学—分子生物学] S565.203[农业科学—作物学]