新疆一枝蒿药材高效液相色谱指纹图谱研究  

Fingerprints of Artemisia rupestris L.from Xinjiang by HPLC

作　　者：贺飞[1,2] 侯桂萍 仲婕[1] 阿布力米提.伊力 阿吉艾克拜尔.艾萨 

机构地区：[1]中国科学院新疆理化技术研究所,乌鲁木齐830011 [2]中国科学院研究生院,北京100039 [3]新疆西域药业有限公司,乌鲁木齐830011

出　　处：《医药导报》2010年第7期854-857,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:20872174);新疆维吾尔自治区重大科技专项项目(基金编号:200733146);新疆维吾尔自治区科技厅少数民族特培项目(基金编号:200723111)

摘　　要：目的研究新疆一枝蒿药材的高效液相色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法色谱柱:Inertsil ODS-3柱(4.6 mm×250 mm,5μm);流动相为甲醇-0.2%的甲酸水溶液,梯度洗脱;流速:1 mL.min-1;检测波长:245 nm;柱温:32℃。结果该方法可使新疆一枝蒿中各成分得到较好的分离,并根据检测结果确定了14个共有指纹峰。结论该指纹图谱检测方法简便、重复性好,可作为新疆一枝蒿药材质量控制的重要方法。Objective To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Artemisia rupestris L.from xinjiang. Methods The sample was separated on an Inertsil ODS-3 column（4.6 mm×250 mm,5 μm） with the mobile phase consisting methanol-0.2% methyl acid by gradient elution;with flow rate at 1.0 mL·min-1,wavelength at 245 nm and the temperature at 32 ℃. Results The chemical constituents of Artemisia rupestris L.were optimally separated,among which 14 fingerprint peaks in common were confirmed. Conclusion This method is simple,accurate and with good reproducibility,which can be used specifically for the quality control of Artemisia rupestris L.from xinjiang.

关 键 词：新疆一枝蒿 一枝蒿酮酸 指纹图谱 色谱法 高效液相 

分 类 号：R282.7[医药卫生—中药学] R927[医药卫生—中医学]