新疆杏品种自交不亲和S-RNase基因特异PCR扩增引物的筛选  

The Specific PCR Primer Selection for S-RNase Gene Associated with Self-incompatibility for Apricot Cultivars in Xinjiang Area

作　　者：姜新[1] 冯建荣[1] 姜俊卿[1] 王大江[1] 刘月霞[1] 

机构地区：[1]石河子大学农学院,新疆石河子832003

出　　处：《新疆农业科学》2010年第6期1061-1065,共5页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30370992);国家科技部"863"计划(2008AA10Z109);国家科技支撑计划(2007BAD36B06)

摘　　要：【目的】筛选鉴定新疆杏自交不亲和相关S-RNase基因的有效引物。【方法】以新疆30个杏品种为试材,选用已报道的蔷薇科李属通用的5对引物组合进行S-RNase基因特异PCR扩增,并依据扩增系数和扩增成功率两个指标对扩增效果进行评价。【结果】(1)5对引物组合对30个杏品种均扩出了条带,除了洛浦2号只扩出1条带之外,其余的29个品种都扩增出了两条带;(2)5对引物组合对新疆主栽杏的扩增效果差异较大,其中EM-PC2consFD+EM-PC3consRD的扩增成功率和扩增系数均为最高(86.67%,95.00%),扩增出两条带的品种有25个;其次,引物组合PaConsⅡ-F+PaConsⅡ-R的扩增成功率达66.67%,扩增系数达83.33%,扩出两条带的品种有20个。【结论】5对引物中EM-PC2consFD+EM-PC3consRD对新疆杏品种S-RNase基因的鉴定效果最好。[ Objective and Method] In order to select the primer for identifying S - RNase gene associated with selfincompatibility of apricots cultivated in Xinjiang, 30 apricot euhivars were used for S - RNase specific PCR with 5 pairs of primer combinations which had been published for prunus consensus. [ Result]The results showed： （ 1 ） The S - RNase pecific bands were amplified in all the 30 cultivars by the 5 pairs of primers, and 29 cuhivars except the ＂Luopu NO.2＂showed two bands amplified. （2） The effect of amplification was significantly different among the 5 pairs of primers. The primer combination of EM - PC2consFD ＋ EM - PC3consRD had the highest amplification coefficient （86.67 % ） and efficiency （95.00 % ）, and two S - RNase specific bands were obtained in 25 cultivars. The amplification coefficient and efficiency of the primer combination PaCons Ⅱ - F ＋ PaCons Ⅱ - R were 66.67% and 83.33% respectively, and two S - RNase specific bands were obtained in 20 cuhivars. [ Conclusion]Therefore, among the 5 pairs of primer combinations, EM - PC2consFD ＋ EM - PC3consRD had the best identification effect for S - RNase gene for Xinjiang apricot cuhivars.

关 键 词：杏 自交不亲和 S—RNase基因 引物筛选 

分 类 号：S662.2[农业科学—果树学] S188[农业科学—园艺学]