花生四烯酸的代谢产物在兔羊水栓塞中的作用  

The effect of arachidonic acid metabolites on pathogenesis of amniotic fluid embolism of rabbit.

作　　者：杨鉴[1,2] 余艳红 潘石蕾[1,2] 钟梅 周凤琴[1,2] 潘志英 

机构地区：[1]第一军医大学南方医院 [2]从化市人民医院

出　　处：《现代妇产科进展》1999年第1期35-38,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金

摘　　要：目的：研究不同性质的羊水注入兔血循环后血中花生四烯酸的代谢产物血栓素Ａ２（ＴＸＡ２）、前列环素Ｉ２（ＰＧＩ２）和白三烯Ｃ４（ＬＴＣ４）的变化。方法：新西兰孕兔２９只，随机分为４组：兔原羊水、胎粪液、胎盘组织提取液和生理盐水，分别在注入前、注入后５ｍｉｎ，、４５ｍｉｎ从兔耳动脉抽血２ｍｌ，用放射免疫分析（ＲＩＡ）检测血中ＴＸＡ２和ＰＧＩ２的代谢物６－酮前列腺素（６－Ｋｅｔｏ－ＰＧＦ１α）和血栓素Ｂ２（ＴＸＢ２），用酶联免疫法（ＥＩＡ）检测ＬＴＣ４。结果：注入胎粪液、胎盘组织提取液后６－Ｋｅｔｏ－ＰＧＦ１α明显增加（Ｐ＜０．０１），兔原羊水组轻度增加，但差异无显著性（Ｐ＞０．０５）；生理盐水组无明显变化（Ｐ＞０．０５）；除生理盐水组外，其余各组的ＴＸＢ２和ＬＴＣ４在注入后增加显著（Ｐ＜０．０１）；ＴＸＢ２、６－Ｋｅｔｏ－ＰＧＦ１α和ＬＴＣ４的组间对比以胎盘组织提取液组增加显著（Ｐ＜０．０１）。结论：羊水进入兔血循环能刺激机体产生ＴＸＡ２、ＬＴＣ４和ＰＧＩ２等花生四烯酸的代谢产物，在羊水栓塞的发生、发展过程中起重要作用。Objective:To study the changes of arachidonic acid metabolites prostaglandin I 2(PGI 2),thromboxane A 2(TXA 2)and leukotriene C 4(LTC 4)in blood during experimental amniotic fluid embolism in rabbits.Methods:Twenty nine New Zealand white rabbits in pregnant 25～31 day were divided into four groups at random:raw amniotic fluid(8 rabbits);cell free filltrate of placental extract(8 rabbits);meconium stained fluid(8 rabbits)and control NS(5 rabbits).The concentrations of TXB 2 and 6 Keto PGF 1α in blood at different time(5min,45min)after injected the substances were measured.Results:The levels of 6 Keto PGF 1α were not elevated remarkable in groups of rabbit raw amniotic fluid and control NS(P>0 05)but the groups of meconium stained fluid and cell free filtrate of placenta increased obviously(P<0 05,P<0 01) The production of TXB 2 and LTC 4 increased except the group of NS(P<0.01).The levels of TXB 2, 6 Keto PGF 1α and LTC 4 in cell free filtrate of placenta were higher than that of other groups(P<0 01) Conclusion:Amniotic fluid of different character stimulate the biosynthesis and release of arachidonic acid metabolites in body and it may play an important role in genesis and development of amniotic fluid embolism.

关 键 词：羊水栓塞 血栓素A2 白三烯C4 花生四烯酸 

分 类 号：R714.468[医药卫生—妇产科学]