荧光定量PCR方法检测IBV病毒在CEK细胞中的动态变化  

Study on Dynamic Change of IBV in Chick Embryo Kidney Cell by FQ-PCR

作　　者：张颖[1] 刘澜澜[1] 王占锋[1] 尹鑫[1] 魏萍 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《现代畜牧兽医》2010年第7期64-68,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：黑龙江省教育厅科学技术研究项目(2003fz012);东北农业大学创新团队课题(cxt006-4-1)

摘　　要：为揭示鸡传染性支气管炎病毒(IBV)在鸡胚肾细胞(CEK)中复制增殖的动态变化规律,通过实时荧光定量PCR(FQ-PCR)检测IBV病毒感染CEK3、6、12、24、36、48、72h后病毒增殖量的动态变化。结果显示,随着时间增加,IBV病毒增殖出现规律性变化,3～12h时IBV增殖不明显,36h时IBV开始大量复制,48h时达到高峰,但72h后有所下降。试验表明IBV在细胞内复制增殖的最佳时间在接毒后48h,其复制增殖情况与细胞状态负相关。To elucidate the dynamic change of IBV in chick embryo kidney cell, detected IBV virus in CEK cells by FQ-PCR after virus infected cells 3, 6, 12, 24, 36, 48 and 72 h. The results show that proliferation of IBV display regularity change with the increase of time. At 3-12h the proliferation is not obvious; at 36 h, IBV increase rapidly; 48 h reach the replicate peak time but after 72 h the viral RNA load decrease slightly. The results reflected that the best time of virus proliferation in CEK cells is infected 48 h and the condition is negative correlation with the growth state of cells.

关 键 词：荧光定量PCR IBV病毒 动态变化 

分 类 号：S852.659[农业科学—基础兽医学]