产KPC酶肠杆菌科细菌对碳青霉烯类抗菌药物的耐药性分析  

Carbapenem resistance of KPC-possessing Enterobacteriaceae

作　　者：洪晓平[1] 杨青[2] 

机构地区：[1]国家电网公司职业病防治院检验科,浙江省建德市311600 [2]浙江大学医学院附属第一医院检验科

出　　处：《中华临床感染病杂志》2010年第3期145-147,共3页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的 评价临床常用的5种药物敏感性（药敏）试验方法对KPC酶介导碳青霉烯类抗菌药物耐药性的检出率.方法 收集2007年10月-2008年9月浙江大学医学院附属第一医院36株经PCR证实产KPC酶的肠杆菌科细菌,包括32株肺炎克雷伯菌、1株大肠埃希菌、1株弗氏枸橼酸杆菌、1株产酸克雷伯菌和1株黏质沙雷菌,用琼脂稀释法、纸片扩散法检测菌株对亚胺培南、美罗培南和厄他培南的耐药性,ATB G-5药敏卡检测亚胺培南、美罗培南的耐药性,BD Phoenix 100NMIC-109药敏卡检测亚胺培南的耐药性,VITEK 2 Compact AST-GN13药敏卡检测亚胺培南、厄他培南的耐药性.结果 ATB、BD Phoenix100、VITEK 2 Compact、琼脂稀释法和纸片扩散法检测亚胺培南耐药率分别为13.9%（5株）、11.1%（4株）、13.9%（5株）、22.2%（8株）和69.4%（25株）;ATB、琼脂稀释法和纸片扩散法检测美罗培南耐药率分别为22.2%（8株）、55.6%（20株）和47.2%（17株）;VITEK 2 Compact、琼脂稀释法和纸片扩散法检测厄他培南耐药率分别为69.4%（25株）、77.8%（28株）和88.9%（32株）.VITEK 2 Compact和琼脂稀释法检测结果显示,厄他培南对所有产KPC酶菌株的MIC均≥2 μg/mL.结论 纸片扩散法和琼脂稀释法能更好地检出KPC酶介导的碳青霉烯类抗菌药物的耐药性,厄他培南可作为筛选产KPC酶菌株的指示药物.Objective To evaluate 5 drug sensitivity tests in detecting carbapenem resistance of KPC-possessing Enterobacteriaceae.Methods Thirty-six Enterobacteriaceae isolates were collected from the First Affiliated Hospital,Cofiege of Medicine,Zhejiang Univeristy,including 32 of Klebsiella pneumoniae,1 of Escherichia coli,1 of Citrobacter freundii,1 of Klebsiella oxytoca,and 1 of Serratia marcescens,and the cacbapenemase producing was confirmed with PCR.Resistance to imipenem,meropenem and ertapenem was determined by agar dilution and disk diffusion tests,that to imipenem and meropehem was determined by ATB with G-5 panel,that to imipenem by BD Phoenix 100 with NMIC-109 panel,and that to imipenem and ertapenem by VITEK 2 Compact with AST-GN13 card.Results The resistance to imipenem determined by ATB,BD Phoenix100,VITEK 2 Compact,agar dilution and disk diffusion tests were 13.9%（5/36）,11.1%（4/36）,13.9%（5/36）,22.2%（8/36）and 69.4%（25/36）.ATB,agar dilution and disk diffusion tests reported 22.2%（8/36）.55.6%（20/36）and 47.2%（17/36）as resistant to meropenem.VITEK 2 Compact,agar dilution and disk diflusion tests reported 69.4%（25/36）,77.8%（28/36）and 88.9%（32/36）as resistant to ertapenem.The MICs of ertapenem determined by VITEK 2 Compact and agar dilution were≥2 μg/mL.Conclusion The sensitivity of agar dilution and agar disk diffusion is higher than other automatic methods in the identification of KPC-mediated carbapenem resistance,and ertapenem is a more sensitive indicator than mempenem and imipenem for screening KPC-possessing isolates.

关 键 词：微生物敏感性试验 碳青霉烯酶 抗药性 

分 类 号：R446.5[医药卫生—诊断学]