荧光PCR技术在粪便幽门螺杆菌ureA基因检测中的应用  

Fluorescence PCR for detecting ureA gene of Helicobacter pylori in feces

作　　者：吴盛海[1] 徐丽慧[1] 余道军[1] 王贤军[1] 

机构地区：[1]杭州市第一人民医院检验科,310006

出　　处：《中华临床感染病杂志》2010年第3期162-165,共4页Chinese Journal of Clinical Infectious Diseases

基　　金：浙江省医药卫生科技计划项目（2007B171）;杭州市医药卫生科技计划重点项目（2007Z001）

摘　　要：目的 评价荧光PCR技术检测粪便幽门螺杆菌（Helicobacter pylori,HP）urea基因的价值.方法 采用荧光PCR技术检测50例消化内科住院患者的粪便样本,其中23例通过胃黏膜活检组织尿素酶及病理组织染色确诊为HP感染.同时进行HP培养和血清HP尿素酶抗体检测.四格表x2检验比较3种方法的敏感性、特异性、阳性预测值和阴性预测值.结果 荧光PCR技术检测粪便HP感染的敏感性、特异性、阳性预测值和阴性预测值分别为1.00、0.96、96%和100%,而HP培养检测的敏感性、特异性、阳性预测值和阴性预测值分别为0.78、1.00、100%和84%,其中敏感性和阴性预测值与荧光PCR法相比,差异具有统计学意义（X2=5.60和4.44,P值均〈0.05）.血清HP尿素酶抗体检测的敏感性、特异性、阳性预测值和阴性预测值分别为0.96、0.74、76%和95%,其特异性和阳性预测值与荧光PCR法比较,差异具有统计学意义（X2=5.28和4.08,P值均〈0.05）.结论 粪便HPureA基因检测对诊断HP感染具有较高的临床价值.Objective To evaluate the application of fluorescence PCR in detecting ureA gene of Helicobacter pylori（HP）in feces.Methods Fluorescence PCR was used to detect ureA gene of HP in feces from 50 patients,including 23 confirmed by gastric biopsy urease test and histological staining.Bacterium culture and serum antibody detection were also performed, and chi-square test was used to compare the sensitivity,specificity,positive predictive value and negative predictive value among three methods.Results The sensitivity,specificity,positive predictive value and negative predictive value of fluorescence PCR were 1.00,0.96,96.O%and 100.0%,while those for HP culture were 0.78,1.00,100.0%and 84.0%,and thee for serum antibody detection Was 0.96,0.74,76.O%and 95.0%.There were significant differences in sensitivity and negative predictive value between PCR and bacterium culture （X2=5.60 and 4.44,P〈0.05）,and significant differences in specificity and positive predictive value between PCR and serum antibody detection（X2=5.28 and 4.08,P〈0.05）.Conclusion ureA gene detection in feces by fluorescence PCR is of value for the diagnosis of HP infection.

关 键 词：螺杆菌 幽门 荧光聚合酶链反应 粪便 诊断 

分 类 号：R4[医药卫生—临床医学] R573