IL-1β和IL-6对椎间盘髓核细胞NGF表达的影响  

Effect of IL-1β and IL-6 on expression of nerve growth factor in human nucleus pulposus cells

作　　者：王刚[1] 韩敦富[2] 施彦璋[3] 陈志维[1] 李逸群[1] 刘尚礼[2] 

机构地区：[1]佛山市中医院骨科,广东佛山528000 [2]中山大学附属第二医院骨科,广东广州510120 [3]汕头市中心医院骨科,广东汕头515031

出　　处：《中国病理生理杂志》2010年第7期1265-1269,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471746)

摘　　要：目的:研究白细胞介素1β(IL-1β)和IL-6对体外培养的椎间盘髓核细胞神经生长因子(NGF)表达的影响。方法:分离培养人腰椎间盘髓核细胞,经过7d的预培养后,实验组分别加IL-1β(10μg/L,50μg/L)和IL-6(10μg/L,50μg/L)进行刺激,对照组加0.3%FBS,48h后应用免疫组织化学法、RT-PCR和Western blotting检测NGF的表达。结果:培养的椎间盘髓核细胞经鉴定为类软骨细胞。对照组髓核细胞很少表现NGF免疫活性,IL-1β和IL-6刺激组明显上调了髓核细胞NGF mRNA及蛋白质的表达,在相同的浓度下IL-6的上调作用更明显。结论:正常情况下NGF在椎间盘髓核细胞呈低表达状态,IL-1β和IL-6能促进髓核细胞NGF的表达,相同浓度下IL-6刺激髓核细胞分泌NGF的作用更强。AIM ： To determine the effect of interleukin - 1β （ IL -1β） and IL - 6 on the expression of nerve growth factor （NGF） by human nucleus pulposus （NP） cells. METHODS： The NP cells from the normal disc of operative patients with scoliosis were isolated, cultured and identified. After 7 days precuhure, the NP cells were treated with IL - 1β （ 10 μg/L, 50μg/L） or IL -6 （ 10 μg/L, 50 μg,/L） for 48 h in the experimental groups and 0.3% PBS was used in the control groups. The expression of NGF was detected by the methods of immunohistochemistry, semi - quantitative RT - PCR and Western blotting. RESULTS ： The NP cells were chondrocyte - like cellular morphology with positive staining of toluidine blue, safranine O and anti - collagen II antibody. The NP cells cultured in monolayer showed immunoreactivity to NGF either in control condition or in experimental group. IL -1β and IL - 6 up - regulated the mRNA expression of NGF and the protein production of NGF. The effect of this up - regulation was higher by treating with IL - 6 than by treating with IL -1β in the same concentration. CONCLUSION： Our results demonstrate that the proinflammatory cytokines IL-1β and IL -6 stimulate the production of NGF in NP ceils. The effect of IL -6 is more significant than that of IL -1β.

关 键 词：白细胞介素1 白细胞介素6 神经生长因子 髓核细胞 

分 类 号：R681.53[医药卫生—骨科学]