促红细胞生成素对创伤性脑损伤大鼠认知功能影响的实验研究  

Effect of erythropoietin on cognitive function in rats with traumatic brain injury

作　　者：贾强[1] 只达石[2] 黄慧玲[2] 武俏丽[2] 王琼[2] 张学斌[2] 常小丽[2] 

机构地区：[1]天津医科大学第二医院神经外科,天津300211 [2]天津环湖医院神经外科,天津300060

出　　处：《中华神经医学杂志》2010年第7期686-689,696,共5页Chinese Journal of Neuromedicine

基　　金：天津市科技发展计划（05YFGDSF02500）

摘　　要：目的 评价促红细胞生成素（EPO）对脑外伤模型大鼠认知功能的作用,并探讨其影响机制.方法 48只雄性SD大鼠按随机数字表法分为对照组、假手术组、模型组和EPO治疗组.后2组建立液压冲击大鼠颅脑损伤模型,假手术组接受同样的操作但不接受液压冲击,对照组未经任何处理.伤后除EPO治疗组立即腹腔注射EPO（5000 U/kg）2 d外,另外3组同一时间腹腔注射等剂量生理盐水.于外伤后30 d应用Morris水迷宫检测大鼠认知功能,伤后37 d应用免疫组化检测脑组织中脑源性生长因子（BDNF）的表达.结果定位航行实验结果显示训练后2、3、4、5 d各组大鼠寻找平台的潜伏期不同,对照组及假手术组潜伏期最短,模型组最长,EPO治疗组介于二者之间,差异有统计学意义（P〈0.05）;空间搜索实验结果显示各组大鼠在原来平台所在象限游泳时间的百分比不同,对照组及假手术组游泳时间的百分比最高,模型组最低,EPO治疗组介于二者之间,差异有统计学意义（P〈0.05）;免疫组化染色结果显示EPO治疗组大鼠脑组织BDNF的表达高于另外3组,差异有统计学意义（P〈0.05）.结论 液压冲击造成的颅脑损伤可损害大鼠的认知功能,外源性给予EPO可以改善外伤后大鼠的空间学习记忆能力,这可能与EPO促进BDNF的表达有关.Objective To evaluate the effect of erythropoietin （EPO） on cognitive function in rats with traumatic brain injury （TBI） and investigate its mechanisms. Methods Forty-eight SD rats were equally randomized into control, sham-operated, TBI and EPO＋TBI groups. TBI models were established in the later 2 groups by operation and hydraulic shock. Sham-operated group received the operation only. The EPO＋TBI group was injected with EPO immediately after the success of model making; the other groups were injected with saline at the same time. On the 30~ d of injury, Morris water maze was employed to evaluate the cognitive function of the rats and the expression of brain-derived growth factor （BDNF） was detected by immunohistochcmistry. Results In the navigation experiment, the latent period （the rats on founding the platform） in the control and sham-operated groups was significantly shorter than that in the other 2 groups （P〈0.05）; that in the TBI group was statistically longer than that in the EPO＋TBI group （P〈0.05）. In the space searching experiment, the swimming times of the rats in each quadrant were significantly different （P〈0.05）： the control and sham-operated groups were the longest and the TBI group was the shortest of all the 4 groups. Immunohistochemistry showed that the expression of BDNF in the EPO＋TBI group was significantly higher than that in the other 3 groups （P〈 0.05）. Conclusion TBI can damage cognitive function of the rats, while exogenous EPO may improve their memory abilities by up-regulating the expression of BDNF.

关 键 词：促红细胞生成素 颅脑损伤 认知功能 脑源性生长因子 

分 类 号：R651.15[医药卫生—外科学]