小麦茎尖丛生芽诱导及植株再生  

Induction and Regeneration of Multiple Shoots from Elite Wheat Cultivars

作　　者：张杰[1] 李和平[1,2] 廖玉才[1,3] 瞿波[1,3] 

机构地区：[1]华中农业大学麦类作物分子生物技术实验室,武汉430070 [2]华中农业大学生命科学技术学院,武汉430070 [3]华中农业大学植物科学技术学院,武汉430070

出　　处：《华中农业大学学报》2010年第4期403-407,共5页Journal of Huazhong Agricultural University

基　　金：国家新品种培育专项(2008ZX08002-001);国家"863"高技术研究发展专项(2007AA100505);教育部博士点基金(20070504010)资助

摘　　要：为了筛选不受季节限制的小麦遗传转化受体,研究了3个小麦栽培品种在3种萌发培养基、4种诱导培养基、3种不同诱导时间和3种生根培养基下从茎尖直接诱导丛生芽的最佳条件和方法。结果表明,MB5基本培养基不添加任何激素最适于小麦成熟胚萌发;而在MB5培养基中添加1.0 mg/L TDZ和0.5 mg/L IBA,小麦茎尖丛生芽诱导率最高;单个茎尖形成丛生芽的数目,随诱导时间延长而增加,诱导时间以不超过30 d为宜;品种间的丛生芽诱导率及丛生芽形成数目没有差异;最佳生根培养基为1/2 MS基本培养基添加1.0 mg/LIBA。由丛生芽诱导再生发育形成的植株开花结实正常。这些研究结果为建立基于小麦丛生芽的再生及遗传转化体系提供了方法。To develop a wheat genetic transformation system that is not restricted by seasons,this work investigated the optimized conditions and methods for induction and regeneration of multiple shoots directly derived from shoot apical meristem tissues in wheat.The study included the comparisons of three germination media,four induction media,three induction times and three rooting media with three elite wheat cultivars.The results indicated that MB5 basal medium without any hormones was suitable for germination of wheat mature embryos.MB5 basal medium plus 1.0 mg/L TDZ and 0.5 mg/L IBA produced the highest rate of multiple shoot induction.Numbers of multiple shoots increased with the increase of induction time.The suitable time for multiple shoot induction is not more than 30 days.There was no difference among the wheat cultivars in terms of induction rate and number of multiple shoots.The best medium for rooting was 1/2 MS basal medium plus 1.0 mg/L IBA.Regenerated plants derived form multiple shoots showed normal flowering and seeds.These results provide useful methods for regeneration and genetic transformation based on multiple shoots of wheat.

关 键 词：诱导 丛生芽 成熟胚 顶端分生组织 小麦 

分 类 号：S512.103.53[农业科学—作物学]