钙化裂须蓝细菌schetR基因超表达菌株的构建及表型分析  

Construction and Phenotype Analysis of Over-Expression Strain of Schizothrix schetR Gene

作　　者：胡璠[1] 王莉[1] 陈雯莉[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2010年第4期460-464,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30670046)资助

摘　　要：为研究在不分化异形胞的钙化裂须蓝细菌Schizothrixcalcicola中schetR基因的功能,本研究构建由强启动子petE驱动的schetR基因的表达质粒,通过三亲本接合转移的方法将此表达质粒转入模式生物鱼腥蓝细菌AnabaenaPCC 7120的hetR突变体中进行功能验证。结果显示:超表达schetR能够互补鱼腥蓝细菌hetR突变体的表型,这说明schetR能够促进异形胞的发育。To investigate the function of schetR gene in a non-heterocystous cyanobacterium,Schizothrix calcicola,the schetR over-expression vector driven by strong promoter petE was constructed and transformed into hetR mutant in model strain Anabaena PCC 7120.The results showed that the over-expression of schetR could complement the phenotype of Anabaena hetR mutant.This indicates that schetR performs a function of stimulating heterocyst development.

关 键 词：钙化裂须蓝细菌 鱼腥蓝细菌 异形胞发育 hetR schetR基因 

分 类 号：Q939.9[生物学—微生物学]