猪MX1基因第8内含子多态性及与生产性状的关联分析  

Polymorphism of Intron 8 of Porcine MX1 Gene and Its Association Analysis with Productive Traits

作　　者：武华玉[1,2] 李良华[1,2] 彭先文[1,2] 宋忠旭[1] 冯政[1] 梅书棋[1,2] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430064 [2]动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《华中农业大学学报》2010年第4期475-478,共4页Journal of Huazhong Agricultural University

基　　金："十一五"国家科技支撑项目(2006BAD01A08;2008BADB2B02);国家生猪现代产业体系建设专项项目(NYCYTX-009);湖北省农业科技创新中心项目(2007-602-001-03);动物胚胎工程及分子育种湖北省重点实验室开放课题(2010ZD140-149)资助

摘　　要：对猪MX1基因第8内含子进行了克隆、测序,结果发现在167 bp处发现1个G/A突变,引起HpaⅡ酶切多态。利用PCR-RFLP方法对国内外6个猪种MX1基因第8内含子的多态性进行分析,发现在大白、长白、通城、清平、鄂西黑猪、大梅F2代等6个品种中等位基因B的基因频率分别为0.83、0.89、0.94、0.87、0.89和0.55;在180头大梅F2代资源家系中进行性状关联分析发现MX1基因第8内含子的多态性与屠宰率、瘦肉率、眼肌高显著关联,屠宰率、眼肌高加性效应显著;眼肌高、瘦肉率显性效应显著。Cloning and sequencing of the eighth intron of porcine MX1 gene indicated that a G167A substitution which can be detected by restriction endonuclease HpaⅡ.This polymorphism was analyzed with PCR-RFLP among six foreign and Chinese pig breeds.The analysis results showed that frequencies of allele B in six pig breeds was 0.83,0.89,0.94,0.87,0.89,0.55 in Large White,Landrace,Tongcheng,Qingping,Exi black pig,Large White ×Meishan F2 progeny pigs respectively.Statistical analysis in 180 Large White ×Meishan F2 progeny pigs showed that the HpaⅡ polymorphism in the F2 population was significantly associated with dressing percentage,lean meat percentage,and loin eye height.Additive effects of dressing percentage,loin eye height were significant.Dominance effect of height and lean meat percentage was significant.

关 键 词：猪 MX1 PCR-RFLP 关联分析 

分 类 号：S828.2[农业科学—畜牧学]