HPLC法流动相选择及相浓度比例对头孢克肟分散片含量测定分析  

Influence of mobile phase and the proportion of phase concentration on the determination of cefixime dispersible tablets in high performance liquid chromatography

作　　者：曾荣华[1] 卢慧娴[1] 邓雪媚[1] 

机构地区：[1]广东肇庆市第一人民医院,广东肇庆526021

出　　处：《海南医学院学报》2010年第7期827-829,共3页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020100234)~~

摘　　要：目的:建立高效液相色谱法测定头孢克肟分散片的含量及有关物质的方法。方法:色谱柱:LunaC18(250mm×4.6mm,5μm),四丁基氢氧化铵溶液∶乙腈(70∶30)为流动相,检测波长为254nm。流速1.0mL/min,进样量:10μL,柱温:40℃。结果:头孢克肟在进样量25～300mg/L的范围内,线性关系良好;回归方程:Y=3.65×104X-1.25×104,r=0.9998。有关物质在进样量150～1500mg/L的范围内线性关系良好;回归方程:Y=578×103X-36×103,r=0.9998。结论:本法试剂易得,样品处理简便,分析快速准确,重现性好,可以作为头孢克肟分散片的含量及有关物质测定方法。Objective： To establish an high performance liquid chromatography （HPLC） for quantitative determination of dispersible tablets and related substances. Methods：Cefixime was separated on ODS column with mobile phase of tetrabutylammonium hydroxide solution..acetonitrile （70.30） and detected at pulse detection of 254 nm. The flow rate was 1.0 mL/min, and the column temperature was 40℃; Injection volume was 10μL Results： The standard curve was linear within the range of 254300 rag/ I. and the regression equation was. Y=3.65×10^1X-1.25×10^3r= 0.9998 in cefixime. The standard curve was linear within the range of 150-1500 mg/ L and the regression equation was Y=S78×10^3X-36 × 10^3 and r= 0.9998 in the related substances. Conclusions：The above mentioned method is simple,rapid,accu- rate,and reliable, thus can be used for quantitative determination of dispersible tablets and related substances.

关 键 词：头孢克肟 分散片 高效液相色谱法 含量 

分 类 号：R927[医药卫生—药学]