新鲜血比对方法在血液分析仪中的应用  

Application of the comparison of fresh whole blood in hematology analyzer

作　　者：任为[1] 陈玮[1] 

机构地区：[1]湖南省人民医院检验科,湖南长沙610007

出　　处：《海南医学院学报》2010年第7期941-943,950,共4页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020100268)~~

摘　　要：目的:应用正常人新鲜全血对多台血液分析仪进行比对,使血液分析仪各参数之间具有可比性。方法:选择性能良好、规范化操作的血液分析仪作为参比仪,用该仪器定值的健康人新鲜血校准比对3台血液分析仪(比对仪)。结果:3台比对仪白细胞(WBC)比对前偏倚分别为(-7.32、-4.49、-8.70)%,血小板(PLT)比对前偏倚分别为(-4.75、-17.1、-11.8)%;WBC比对后偏倚分别为(-0.3、2.5、-1.7)%,PLT比对后偏倚分别为(-1.7、-0.7、-1.1)%;WBC、PLT差异有统计学意义(P<0.05),RBC、HGB、HCT、MCV差异无统计学意义(P>0.05),但相关性存在差异,通过比对试验校准仪器后结果一致性良好。结论:该方法能方便、经济地对血液分析进行有效的质量控制。Objective：To investigate application of the comparison of fresh whole blood in hematology analyzer.Methods：The hematology analyzer with good performance,and standardized operation was selected as a reference instrument,and the results tested by 3 hematology analyzers with the fresh blood of healthy individuals was calibrated based on the former.Results：For the 3 hematology analyzers,the bias before the comparison were：WBC（-7.32,-4.49,-8.70）%;RBC（-1.34,-3.58,-1.34）% ;HGB（-0.06,1.81,1.69）%;HCT（3.05,1.52,-0.8）%;MCV（0.03,0.67,4.7）%;PLT（-4.75,-17.1,-11.8）%,respectively;the bias after comparison were：WBC（-0.3,2.5,-1.7）%;RBC（0.9,-1.6,-1.3）%;HGB（1.4,0,-0.03）%;HCT（1.7,-0.2,0.3）%;MCV（0.9,-0.6,-0.3）%;PLT（-1.7,-0.7,-1.1）%.There was significant difference in WBC and PLT（P 〈0.05）,and no significant difference in RBC,HGB,HCT,MCV（（P〉 0.05）.But there was differenct relevance.The results by calibrating the equipment were consistent with the comparison test.Conclusions：The method is effective and economical in quality control for the blood analysis.

关 键 词：血液分析仪 新鲜全血 比对法 

分 类 号：R446.1[医药卫生—诊断学]