二甲戊乐灵离体诱导酸枣八倍体的研究  

A study on octoploid induction of sour jujube(Ziziphus acidojujuba C.Y.Cheng et M.J.Liu) with pendimethalin in vitro

作　　者：路芳[1] 代丽[1] 刘孟军[1] 赵锦[1] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001

出　　处：《河北农业大学学报》2010年第3期42-45,共4页Journal of Hebei Agricultural University

基　　金：河北省杰出青年基金项目(C2010000679);河北省科技支撑计划(06220117D-2);国家科技支撑计划(2008BAD92B03;2007BAD36B07;2006BAD18B02)

摘　　要：以二甲戊乐灵为诱变剂，利用浸泡法对酸枣四倍体的茎段进行离体诱变，并采用流式细胞分析仪鉴定了形态发生变化的植株细胞染色体倍性。结果表明：二甲戊乐灵诱导酸枣八倍体的最适时间和浓度为200～300μmol／L的溶液处理8～10d，诱变率为20．00％。在继代1次后，植株形态发生变化。继代4次后，经流式细胞仪测定为四倍体和八倍体的嵌合体。Pendimethalin was used as mutagen, the shoot segments of the tetraploid plantlet of sour jujube were soaked in solution medium. Ploidy levels were determined by flow cytometry. The results showed that the optimum combination of concentration and time for octoploid induction of sour jujube was 200-300μmol/L pendimethalin for 8-10 days. The doubling effect is 20.00%. The phenotypic variation was produced after one subculture. After four subcul- tures, chimeras of tetraploid and octoploid were identified by the flow cytometry.

关 键 词：酸枣 二甲戊乐灵 八倍体 离体诱变 

分 类 号：S665.1[农业科学—果树学]