乙型肝炎携带者卵巢颗粒细胞中HBV DNA的检测  

Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers

作　　者：梅顺利[1] 黄锦[1] 刘平[1] 张小为[1] 乔杰[1] 张秋芳[1] 张莹[1] 

机构地区：[1]北京大学第三医院妇产科,100191

出　　处：《中华医学杂志》2010年第25期1778-1780,共3页National Medical Journal of China

摘　　要：目的 探讨乙型肝炎病毒在女性乙肝携带者卵巢颗粒细胞中的感染情况.方法 6例女性乙肝携带者（HBsAg＋、HBeAg＋、HBcAb＋）的卵泡液提取颗粒细胞,制成涂片.应用特异性的经地高辛标记的乙肝病毒DNA探针,利用原位杂交检测颗粒细胞涂片.显色反应采用碱性磷酸酶系统,阳性信号呈紫蓝色.结果 6例患者的颗粒细胞中有3例检测到HBV DNA阳性信号,位于细胞核.结论 乙型肝炎携带的卵巢颗粒细胞内存在HBV DNA.女性乙型肝炎携带者存在经生殖细胞向子代传播乙型肝炎的可能性.Objective To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.Methods The foilicular fluid of 6 female carriers of hepatitis B （HBsAg＋,HBeAg＋,HBcAb＋） was used to extract granule cells and prepare smears.The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe.The color reaction system of alkaline phosphates was used.And the positive hybridization signal was purple blue.Results Three granulose cell samples.located at the nuclei of gcanulose cells.were HBV-DNA positive.Conclusion HBV DNA exists in the granulosa cells of hepatitis B patients.Thus HBV infection may be vertically transmitted to the offspring via the infected reproductive cells.

关 键 词：颗粒细胞 乙型肝炎病毒 原位杂交 

分 类 号：R512.62[医药卫生—内科学]