高效液相色谱法测定解毒利尿颗粒中甘草酸含量  

Determination of Glycyrrhizic Acid in Jiedu Liniao Granules by HPLC

作　　者：杜树山[1] 张铮[2] 张海鸣[1] 沈圣民[1] 曹杰[1] 关铭[2] 

机构地区：[1]中药资源保护与利用北京市重点实验室,北京100875 [2]吉林大学药学院,吉林长春130021

出　　处：《时珍国医国药》2010年第7期1613-1614,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支撑计划课题(No.2007BAI48B10);北京市新医药学科群重点支持项目(No.xk100270569)

摘　　要：目的建立解毒利尿颗粒中甘草酸含量的高效液相测定方法。方法采用DiamonsilTM C18柱(250mm×4.6mm,5μm),以甲醇-[0.02mol·L-1醋酸铵-冰醋酸(33∶1)](64∶36)为流动相,流速为1ml.min-1;柱温30℃;检测波长250nm。结果甘草酸在0.15～0.75μg范围内呈良好的线性关系(r=0.9998),该方法平均回收率为98.99%,RSD为1.74%(n=6)。结论该方法简便、稳定易行、准确、重现性较好,结果可靠,可为解毒利尿颗粒的质量评价提供有效依据。Objective To establish a method for determination of glycyrrhizic acid in Jiedu Liniao granules by HPLC.Methods DiamonsilTM C18 column（5 μm,250mm×4.6 mm）was used with methol-（0.02 mol·L-1 ammonium acetate-glacial acetic acid（33∶1））（64∶36）as mobile phase.The flow rate was 1.0 ml·min-1,the column temperature was 30℃ and the detection wavelength was 250nm.Results The calibration curve were linear within the range of 0.15～0.75 μg（r=0.9998）.The average recovery rate was 98.99%（RSD=1.74%）.Conclusion The method is convenient,stable and accurate,so it can be used for determination of glycyrrhizic acid in Jiedu Liniao granules.

关 键 词：高效液相色谱法 解毒利尿颗粒 甘草酸 

分 类 号：R284.1[医药卫生—中药学]