氧耗剂致乳鼠心肌缺氧/复氧模型的建立  

Establishment of Hypoxia/Reoxygenation Model in Neonatal Rat Cardiomyocytes by Oxygen Agent

作　　者：余薇[1] 许波[1] 彭彦[1] 吴基良[1] 李璐[1] 

机构地区：[1]咸宁学院.药学院,湖北咸宁437100

出　　处：《时珍国医国药》2010年第7期1660-1661,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省教育厅资助项目(No.Q20082805)

摘　　要：目的探讨利用氧耗剂建立乳鼠心肌缺氧/复氧损伤模型的一种新方法。方法取SD乳鼠原代心肌细胞培养,观察氧耗剂连二亚硫酸钠不同浓度(1,2,3,4,5,6mmol/L)分别缺糖缺氧1h复氧24h及在4mmol/L浓度下缺糖缺氧不同时间(5min,15min,30min,1h,2h,4h,6h)后复氧24h对心肌细胞存活率及培养上清液中乳酸脱氢酶(LDH)的影响,并进行细胞形态学观察。结果乳鼠心肌细胞经过缺氧/复氧处理后,与正常对照组相比,连二亚硫酸钠可呈浓度依赖性降低心肌细胞存活率,尤其Na2S2O4浓度大于3mmol/L时细胞MTT的OD值与正常对照组有显著性差异,同时上清液中LDH含量升高,差异具有统计学意义(P<0.05或P<0.01)。而当连二亚硫酸钠4mmol/L浓度下随着缺氧时间延长,细胞的存活率显著降低,而心肌细胞上清液中LDH的释放量显著升高,差异具有统计学意义(P<0.05或P<0.01),呈现时间依赖性。结论应用连二亚硫酸钠可建立心肌细胞缺氧再灌注损伤模型。Objective To establish hypoxia/reoxygenation model in neonatal rat cardiomyocytes by oxygen agent.Methods Cultivate neonatal rat cardiomyocytes,observe the apoptotic cardiac myocyte by using MTT assay and after being given Na2S2O4 with different concentration（1,2,3,4,5,6 mmol）for 1h and then reoxygenation for 24h;besides give Na2S2O4 with 4 mmol/L at different time（5,15,30 min,1,2,4,6 h）and then reoxygenatie for 24 h.Biochemical method was used to determine lactate dehydrogenase（LDH）activity.Results Na2S2O4 could increase cell apoptosis and the level of lacate dehydrogenase（LDH）the supernatant fluid.Conclusion It is an effective hypoxia/reoxygenation mode of using Na2S2O4 in neonatal rat cardiomyocytes.

关 键 词：心肌细胞 缺氧 复氧 细胞模型 连二亚硫酸钠 

分 类 号：R331[医药卫生—人体生理学]