大豆黄酮和雌二醇体外灌流公猪垂体组织对GnRH诱导下LH分泌的影响  

Effects of daidzein and estradiol on GnRH induced LH in vitro release from the pituitaries of male pigs in the perfusion system

作　　者：王根林[1,2,3] 陈杰[1,2,3] N.Parvizi 

机构地区：[1]南京农业大学动物科技学院 [2]南京农业大学农业部动物生理生化重点开放实验室 [3]德国农业研究中心动物科学与动物行为研究所

出　　处：《南京农业大学学报》1999年第1期65-68,共4页Journal of Nanjing Agricultural University

摘　　要：用不同浓度的大豆黄酮（Ｄａ，处理Ⅰ３９４ｎｍｏｌ／Ｌ，处理Ⅱ７８７４ｎｍｏｌ／Ｌ）和雌二醇（Ｅ２，处理Ⅲ１００ｐｍｏｌ／Ｌ，处理Ⅳ２００ｐｍｏｌ／Ｌ）体外灌流公猪垂体前叶组织，观察其对促黄体素（ＬＨ）分泌的影响。灌流２４和４８ｈ后，Ｄａ和Ｅ２灌流组与对照组（处理Ⅴ）相比，垂体ＬＨ体外分泌水平都无明显差异。但在促性腺激素释放激素（ＧｎＲＨ）诱导下，各组ＬＨ分泌水平显著增加，诱导后２０～３０ｍｉｎ出现分泌峰。ＧｎＲＨ诱导后１ｈ，ＬＨ平均水平处理Ⅲ显著高于其他组（Ｐ＜０．０５或Ｐ＜０．０１）；诱导后２ｈ，处理Ⅱ和Ⅲ显著高于对照组（Ｐ＜０．０１），处理Ⅲ亦高于处理Ⅰ（Ｐ＝０．０５）；诱导后３ｈ，各组ＬＨ分泌无显著差异。结果表明，Ｄａ和Ｅ２对公猪垂体体外ＬＨ分泌无抑制作用，但对ＧｎＲＨ诱导的ＬＨ分泌有一定促进作用。Daidzein(Da) and estradiol benzoate(E 2)were applied to perfuse anterior parts of the pituitaries of Goettingen Miniature male pigs in order to study their effects on LH release in vitro. 24 and 48 h after the perfusion,LH mean levels were not significantly different in Da group(treatment Ⅰ,394 nmol/L;treatment Ⅱ,7 874 nmol/L)and E 2 group(treatment Ⅲ,100 pmol/L; treatment Ⅳ, 200 pmol/L)compared to the control(treatment Ⅴ).LH mean levels in all the groups increased significantly and reached the peaks at 20 to 30 min after GnRH induction.At h 1 after GnRH induction,LH level in treatment Ⅲ was significantly higher than in the other groups( P<0 05 or P <0 01).It was higher in treatment Ⅱ and Ⅲ at h 2 after GnRH induction compared to the control( P<0 01 )and also higher in treatment Ⅲ than in treatment Ⅰ (P=0 05) There were no differences between the groups at h 3 after GnRH induction.The results suggested that Da and E 2 could not inhibit but stimulate GnRH induced LH in vitro release to some extent from the pituitaries of male pigs.

关 键 词：大豆黄酮 雌二醇 体外灌流 垂体 促黄体素 公猪 

分 类 号：S828.1[农业科学—畜牧学]