Acinetobacter sp. XA05和Sphingomonas sp. FG03苯酚生物降解特性研究  

Characterization of phenol biodegradation by acinetobacter sp. XA05 and Sphingomonas sp. FG03

作　　者：刘永军[1] 刘金光[1] 李华[1] 王晓昌[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西部建筑科技国家重点实验室(筹),陕西西安710055

出　　处：《西安建筑科技大学学报（自然科学版）》2010年第3期420-426,共7页Journal of Xi'an University of Architecture & Technology(Natural Science Edition)

基　　金：国家自然科学基金重点项目(50838005);榆林市科技计划项目(Sg09-2-25)

摘　　要：从活性污泥和苯酚污染的土壤中分离出来两个菌株,分别编号为XA05和FG03.通过16S rRNA基因序列分析表明,XA05属于Acinetobacter sp.,而FG03属于Sphingomonas sp..将XA05和FG03在以不同浓度的苯酚作为唯一碳源的基础培养液中培养,结果显示,在初始苯酚浓度分别为800 mg/L和1000 mg/L时,作用45 h和60 h后,XA05和FG03对苯酚的去除率分别是99.5%、78.3%和97.6%、68.1%.两个菌株按1:1的体积比混合后,当苯酚的初始浓度分别为800 mg/L和1000 mg/L时,作用35 h和60 h后,苯酚去除率分别为99.8%,97.2%.XA05和FG03的苯酚降解动力学研究表明,在Haldane’s模型中,XA05和FG03都有较高的KS和KSI值.Two new strains named XA05 and FG03 were isolated from the activated sludge and phenol-contaminated soils,respectively.16S rRNA gene sequences showed that XA05 belonged to an Acinetobacter sp.and FG03 was closely related to the Sphingomonas sp.Strain XA05 and FG03 were cultivated in minimal medium（MM） supplemented with different phenol concentrations as a sole carbon source.Results showed that strain XA05 and FG03 were capable of removing 99.5%,78.3% and 97.6%,68.1% phenol,with an initial concentration of 800 mg/L and 1 000 mg/L phenol in 45 h and 60 h,respectively.When two strains were mixed at the volume ratio of 1：1 for phenol degradation,which gave 99.8% and 97.2% phenol removal ratio within 35 h and 60 h.with an initial phenol concentration of 800 mg/L and 1 000 mg/L,respectively.Kinetics of phenol degradation by strain XA05 and FG03 showed that the values of KS and KSI were high in Haldane′s model.

关 键 词：苯酚 ACINETOBACTER sp. XA05 SPHINGOMONAS sp. FG03 生物降解 动力学 

分 类 号：X703[环境科学与工程—环境工程]