罗汉果查尔酮合成酶基因的生物信息学分析  

Bioinformatics Analysis of the Chalcone Synthase Gene from Luo Han Guo(Siraitia grosvenorii)

作　　者：王志强[1] 蒙姣荣[1] 邹承武[2] 黄锦宽[2] 林纬[3] 黎起秦[3] 陈保善[1,2] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室,南宁530005 [2]广西大学生命科学与技术学院,南宁530005 [3]广西大学农学院,南宁530005

出　　处：《基因组学与应用生物学》2010年第3期577-583,共7页Genomics and Applied Biology

基　　金：广西重点实验室能力建设计划(桂科能05112001-1);广西自然科学基金项目(桂科自0832045)共同资助

摘　　要：查尔酮合成酶(chalcone synthase,CHS)是类黄酮生物合成的关键酶,在植物发育、防止UV损伤、抗病和逆境反应中起着重要作用。本研究通过EST测序,获得了罗汉果查尔酮合成酶基因序列(登录号:GU980155)。为了进一步了解罗汉果查尔酮合成酶基因的特征,我们将其与46种植物的查尔酮合成酶基因的核酸序列和氨基酸序列进行比对和进化分析。结果表明,罗汉果查尔酮合成酶基因的核酸序列和氨基酸序列与其它物种的查尔酮合成酶基因均具较高同源性,编码区相似性约为94%。使用PHYLIP和MEGA4分别构建了邻接树、最大似然树和最大简约树,但经bootstrap检验,最优树未能明确罗汉果查尔酮合成酶基因的系统发育地位。以紫花苜蓿查尔酮合成酶的三维结构为参考,利用同源建模的方法预测了罗汉果查尔酮合成酶的三维结构,发现罗汉果查尔酮合成酶具有保守的活性位点和空间结构。The chalcone synthase（CHS） is a key enzyme in the biogenesis of flavonoids,compounds that playimportant roles in the development,UV damage prevention,disease resistance,and stress response in plant.The sequence of a gene,CHS,encoding the CHS of Luo Han Guo（Siraitia grosvenorii）,was obtained by EST sequencing of the Luo Han Guo cDNA library.BLAST search revealed a similarity of about 94% at amino acid level between the deduced Luo Han Guo CHS and the CHSs of many plant species.A phylogenetic tree using Luo Han Guo CHS sequence and those of 46 plant species was constructed using PHYLIP and MEGA4 programs and it was confirmed by the bootstrap method.However,this tree failed to uncover the evolutionary relationship of Luo Han Guo CHS to the CHS of other species.The three-dimensional（3D） structure of the Han Guo CHS was obtained by homology modeling with the 3D structure of Medicago CHS as reference.Conserved active site and spatial structure were predicted to present in this enzyme as those in the medicago CHS crystal.

关 键 词：罗汉果 查尔酮合成酶基因 进化分析 

分 类 号：S668.9[农业科学—果树学]