应用SPR生物传感器检测酸菜发酵液中大肠杆菌  

Detection of Escherichia coli in Chinese sauerkraut juice using a sur-face plasmon resonance(SPR) biosensor

作　　者：张文浩[1] 郑先哲[1] 初国超[1] 

机构地区：[1]东北农业大学工程学院,哈尔滨150030

出　　处：《东北农业大学学报》2010年第6期117-121,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省重大科技攻关项目(GA07B401-4)

摘　　要：应用SPR传感器可以快速检测酸菜发酵液中的大肠杆菌,以便检测和控制酸菜发酵液中的亚硝酸盐含量,保证发酵食品安全。采用生物素-亲和素系统增大SPR传感器折射信号,获得了传感器的折射率与大肠杆菌浓度的回归方程,实现了对酸菜发酵液中大肠杆菌浓度快速,精确检测,提高大肠杆菌检测限到104cfu·mL-1。An integrated portable Spreeta^TM SPR sensor was used to detect Escherichia coli in Chinese sauerkraut juice, in order to measure and control the content of nitrite in Chinese sauerkraut juice. An avidin-biotin system was employed to amplify the refractive signal of SPR biosensor. The regression equation between the refractive index and concentration was obtained to detect quickly and accurately the content of Escherichia coli in Chinese sauerkraut juice with the high detectability of 10^4 cfu·mL^-1.

关 键 词：等离子共振生物传感器 酸菜发酵液 大肠杆菌 生物素-亲和素系统 检测限 

分 类 号：Q517[生物学—生物化学]