即刻电针同神经节段经穴与非穴对痛经大鼠模型子宫IP_3的影响  

Effect on IP_3 in Uterus Tissue of Dysmenorrhea Model Rats with Instant Electroacupuncture the Same Nerve Segment Acupoint and Non-acupuncture Point

作　　者：丁喜艳[1,2] 任晓暄[1] 李春华[1] 郭孟玮[1] 赵雅芳[1] 嵇波[1] 朱江[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸学院,国家中医药管理局针灸特色疗法评价重点研究室,北京100029 [2]北京市丰台区新村社区卫生服务中心,北京100070

出　　处：《中国中医药信息杂志》2010年第7期37-40,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2006CB504503)

摘　　要：目的观察即刻电针同神经节段经穴与非穴对痛经大鼠模型子宫三磷酸肌醇(IP3)的影响。方法 50只雌性SD大鼠,随机分为盐水组、模型组及电针三阴交组、悬钟组、非穴组,每组10只。除盐水组外,其余各组大鼠均连续10d给予皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素2U/只,制造痛经大鼠模型,盐水组每日给予同等剂量的生理盐水。于第10日各组给予即刻电针20min,盐水组及模型组束缚20min,同时观察扭体反应,并采用酶联免疫法检测子宫IP3含量。结果①扭体反应:与盐水组比较,模型组每分钟扭体次数明显增多、每分钟扭体分数明显升高(均P<0.01);各电针组每分钟扭体次数明显增多(均P<0.01),每分钟扭体分数差异均无统计学意义(均P>0.05)。与模型组比较,各电针组每分钟扭体次数、扭体分数均明显降低(均P<0.01)。各电针组之间每分钟扭体次数、扭体分数差异均无统计学意义(均P>0.05)。②子宫IP3水平:与盐水组比较,三阴交组、模型组、悬钟组、非穴组均明显降低(P<0.05;P<0.01)。与模型组比较,三阴交组IP3含量明显升高(P<0.05);其他电针各组差异均无统计学意义(均P>0.05)。三阴交组子宫IP3明显高于悬钟组(P<0.01)。结论即刻各电针组明显抑制了大鼠扭体反应,且电针各组在改善痛经症状中无明显差异,可能与同神经节段支配有关。三阴交组在对IP3的调节上与悬钟组有明显差异,体现了经穴效应特异性。Objective To research the effect on IP3 in the uterus tissue of dysmenorrhea model rats with instant electroacupuncture （EA） the same nerve segment acupoint and non-acupuncture point. Methods Fifty femal SD rat were randomly divided into saline, model, EA “Sanyinjiao”（SP6）, EA“Xuanzhong”（GB39）, EA non-acupuncture point （NAP） groups with 10 rats in each group. Except for saline group, the rats were given subcutaneous injection of estradiol benzoate injection for 10 days. 1 h after the last administration, intraperitoneal injection of oxytocin 2 U was given to each rat to create dysmenorrhea model, saline group was given the same dose of saline per day. On the 10th day, each group of acupuncture was given EA 20 min, saline group and model group were bound 20 min, the writhing response was observed at the same time. The uterine IP3 content was detected with enzyme-linked immunosorbent assay method. Results ①Writhing response：Compared with the saline group, writhing numbers per minute and writhing scores per minute increased significantly in the model group （all P〈0.01）. Writhing numbers per minute increased significantly （all P〈0.01）, writhing scores per minute had no significant difference in the EA groups （all P〉0.05）. Compared with the model group, writhing numbers and writhing scores per minute decreased significantly in the EA groups （all P〈0.01）. Writhing numbers and writhing scores per minute had no significant difference among the EA groups （all P〉0.05）. ②IP3 content level of the uterus：Compared with the saline group, it was lower in SP6 group （P〈0.05）, and lower significantly in model, GB39 and NAP groups （all P〈0.01）. Compared with model group, SP6 group increased significantly （P〈0.05）, there was no significant difference in the other EA groups （P〉0.05）. The IP3 level in SP6 group was significantly higher than that in GB39 group （P〈0.01）. Conclusion Instant EA groups can inhibit the rats writhing response. EA group

关 键 词：痛经 三磷酸肌醇 非穴 经穴效应特异性 即刻电针 大鼠 

分 类 号：R285.5[医药卫生—中药学]