多因素复合诱变选育克拉维酸高产菌株  

Breeding of Clavulanic Acid High-producing Strains by Multiple Mutagens Induction

作　　者：田敏[1] 黄运昌 朱春燕 朱辉 余蓉[1] 

机构地区：[1]四川大学华西药学院 [2]成都雅途生物技术公司,四川成都610041

出　　处：《中国医药工业杂志》2010年第7期501-503,共3页Chinese Journal of Pharmaceuticals

基　　金：国家科技部创新基金资助项目(06C26225100494)

摘　　要：采用多因素连续复合诱变的方法,对克拉维酸产生菌带小棒链霉菌(Streptomyces clavuligerus)进行系列诱变,以抗生素抗性作为理性筛选标记,从含青霉素35μg/ml的琼脂平板上筛选到青霉素抗性突变株YT-201-57#,在摇瓶实验中,对青霉素的抗性由8μg/ml提高到45μg/ml,克拉维酸产量较出发菌株提高10.3%,且高产遗传特性经5次传代仍稳定。Some mutants were obtained after multiple mutagenesis of a clavulanic acid-producing strain Streptomyces clavuligerus YT-201,among which a penicillin resistance mutant named as YT-201-57^# was selected on the agar plates containing 35 μg/ml penicillin.The resistant level of this mutant against penicillin was increased from 8 μg/ml to 45 μg/ml.Its yield of clavulanic acid was also increased by 10.3% in flask fermentation compared with parent strain.The production level of this mutant is stable after 5 continuous generations of passage.

关 键 词：克拉维酸 复合诱变 抗性筛选 突变株 

分 类 号：Q933[生物学—微生物学]