取代度相同的N-酰化低聚壳聚糖清除自由基作用的研究  

The Radical Scavenging Effect of N-Acyl Chitosan Oligosaccharide

作　　者：孙涛[1] 银旭红[1] 谢晶[1] 李立[1] 赖克强[1] 周冬香[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《食品与生物技术学报》2010年第3期406-409,共4页Journal of Food Science and Biotechnology

基　　金：上海市教育委员会科研项目(07zz134);上海市重点学科建设项目专项基金(T1102);上海市生物医药和农业科技领域重点科技项目(08391911500);2009年上海市优秀学科带头人计划(09XD1402000)

摘　　要：低聚壳聚糖经N-酰化得到取代度相同的N-马来酰低聚壳聚糖和N-邻苯二甲酰低聚壳聚糖,其取代度均为0.25。用红外光谱对其结构进行表征,凝胶色谱测定其相对分子质量大小。并考察了其对羟基自由基(.OH)和1,1-二苯基苦基苯肼自由基(DPPH)的清除能力。结果表明:取代度相同,N-邻苯二甲酰低聚壳聚糖对.OH、DPPH的清除能力优于N-马来酰低聚壳聚糖。这说明取代度相同时,取代基的结构会影响N-酰化低聚壳聚糖对自由基的清除活性。N-maleyl chitosan oligosaccharide （NMCOS） and N-phthaloyl chitosan oligosaccharide （NPCOS） with the same substituting degrees （0.25） were synthesized.The structure were characterized by FTIR spectra,the molecular weight was measured by Gel Permeation Chromatog-raphy （GPC）.Their antioxidant activity were evaluated by the scavenging of hydroxyl radical （.OH） and 1,1-Diphenyl-2-picrylhydrazyl radical （ DPPH）.The results showed NPCOS have stronger hydroxyl and DPPH radical scavenging activity than that of NMCOS.The results presented here demonstrated that the antioxidant activity of N-acyl Chitosan Oligosaccharide with the same substituting degrees was determined by the substituting groups.

关 键 词：低聚壳聚糖 N-酰化 抗氧化 取代度 

分 类 号：R341[医药卫生—基础医学]