阿是止泻胶囊对甲状腺功能亢进性腹泻大鼠杯状细胞的影响  

Effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrheic rats

作　　者：张瑛琦[1] 郭敏[1] 乔国芬[2] 成志锋[1] 

机构地区：[1]哈尔滨医科大学附属第四医院内分泌科,150001 [2]哈尔滨医科大学药学院黑龙江省生物医药重点实验室,150001

出　　处：《中国地方病学杂志》2010年第4期375-378,共4页Chinese Jouranl of Endemiology

摘　　要：目的 探讨阿是止泻胶囊对甲状腺功能亢进性腹泻（简称甲亢性腹泻）大鼠胃肠道杯状细胞的影响.方法 8周龄的雄性SD大鼠120只,按体质量随机分为对照组（10只）和甲亢性腹泻组（110只）.对照组按每只大鼠1ml/d生理盐水灌胃 其余大鼠给予40g/L的甲状腺片混悬液,每只大鼠1 ml/d.根据大鼠血清FT3、FT4水平、体质量和湿粪的数量筛选出40只甲亢性腹泻大鼠.将甲亢性腹泻大鼠按体质量随机分为腹泻对照组、黄连素组,阿是止泻胶囊低剂量组、中剂量组、高剂量组,每组8只.腹泻对照组：按1 ml/d生理盐水给药 黄连素组：按1.94 g·kg-1·d-1给黄连素片 低、中、高剂量组：分别按0.63、1.26、2.52 g·kg-1·d-1给予阿是止泻胶囊.各组连续灌胃7 d,次日脱颈处死,取十二指肠、空肠、回肠、结肠组织,HE染色,光镜观察组织形态及杯状细胞的计数.细胞计数单位为细胞数/高倍镜下每视野（cells/hpf）.结果 在空肠中,除低剂量组[（23.98±2.28）cells/hpf]外,黄连素组、中剂量组、高剂量组杯状细胞数量[（15.32±2.53）、（20.24±1.24）、（14.98±1.10）cells/hpf]均低于腹泻对照组[（25.73±4.55）cells/hpf,P均〈0.05] 结肠中,黄连素组、低剂量组、中剂量组、高剂量组结肠杯状细胞数量[（18.29 ±1.33）、（20.61±2.12）、（19.38±2.01）、（16.34±1.55）cells/hpf]均低于腹泻对照组[（23.36±3.10）cells/hpf,P均〈0.05].空肠和结肠腹泻对照组和高剂量组均低于低剂量组（P均〈0.05）.十二指肠和回肠中杯状细胞数量组间比较差异无统计学意义（F=2.81、2.67,P均〉0.05）.形态学观察显示,空场和结肠组织中,腹泻对照组杯状细胞数量明显增多,而随着治疗剂量的增加细胞数量逐渐减少.结论 甲亢性腹泻大鼠空肠和结肠组织中杯状细胞数量明显增多,阿是止泻胶囊能显著降低空肠和结肠组织中杯状细胞数量,减少黏液分泌.Objective To study the effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrhea.Methods Total of 120 SD male rats aged about 8 weeks were randomly divided into 2 groups：control group（10 rats）and thyroid hormone-induced diarrheic group（110 rats）.Rats in control group were lavaged with normal saline 1 ml/d.Thyroid tablets were partly desolved into normal saline forming a 40 mg/ml suspension.Rats in thyroid hormone-induced diarrheic group were given the thyroid suspension 1 ml/d to make thyroid hormone-induced diarrheic model.Serum FT3 and FT4 were tested.Fourty thyroid hormone-induced diarrheic rats were screened out according to serum FT3 and FT4 levels,body weight and wet stool.The fourty rats were randomly divided into 5 groups,8 rats in each group：positive control group,berberine group,low-dose,mediandose and high-dose groups.Normal saline of 1 ml/d was admnistered to diarrhea control group,1.94 g·kg-1·d-1 Berberine capsule was given to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 Asi-antidiarrheal capsule to low-dose,mediandose and high-dose groups,respectively.After sever days treatment,rats are executed.Duodenum,jejunum,ileum and colon were dissected,respectively.Histology observation and cell counting were carried out under light micmscopo on HE coloration.Cell counting unit was defined as：cell/high power field of vision （cells/hpf）.Results In jejunum,the number of goblet cells in berberine group,mediandose group and high-dose group[（15.32±2.53）,（20.24±1.24）,（14.98±1.10）cells/hpf,respectively],were all lower than that of the diarrhea control group[（25.73±4.55）cells/hpf,all P〈0.05]with an exception of low-dose group[（23.98±2.28）cells/hpf].The numbers of goblet cells in berberine control group,low-dose group,mediandose group and highdose group[（18.29±1.33）,（20.61±2.12）,（19.38±2.01）,（16.34±1.55）cells/hpf,respectively]were all less than that of the control group[（23.36±3.10）cells/hpf,

关 键 词：甲状腺功能亢进症 腹泻 阿是止泻胶囊 杯状细胞 

分 类 号：R686[医药卫生—骨科学]