黄芪复合剂抗血吸虫作用的实验研究  

Experimental Study of Astragalus Combining Forms on Schistosomicidal Effect

作　　者：邹艳[1,2] 丘继哲[2] 曾庆仁[1] 蔡力汀[1] 刘碧源[1] 魏琦[1] 

机构地区：[1]中南大学基础医学院细胞与分子生物学实验中心,长沙410013 [2]湖南中医药高等专科学校基础医学部,株洲412012

出　　处：《热带医学杂志》2010年第6期654-656,690,F0004,共5页Journal of Tropical Medicine

基　　金：湖南省卫生厅资助项目(No.B2008-068)

摘　　要：目的比较黄芪、南瓜子仁和槟榔单一与联用抗血吸虫的效果差异。方法实验设感染对照、感染后1～10d用复合中药、感染后8～17d用复合中药、感染后15～24d用复合中药、感染后28～37d用复合中药、感染后1～10d单用黄芪组、感染后1～10d单用南瓜子、感染后1～10d用南瓜子+槟榔、感染后36d用300mg/kg·d的吡喹酮(PZQ)等9个组,观察以黄芪为主药所组成的中药复合剂对感染小鼠体内不同发育期虫体作用的差异。结果与平行的感染对照组相比,复合中药用于血吸虫感染后1～10d组、8～17d组和15～24d组的减虫率,分别为36.21%、26.74%和39.04%,肝卵减少率分别为58.6%、32.2%和47.7%,差异均具有统计学意义(P<0.05),且肝表面虫卵结节密度值可见明显减小。在感染后28～37d复合中药组、感染后1～10d单用黄芪、单用南瓜子组和南瓜子+槟榔合用组与平行的感染对照组比较,减虫率和肝卵减少率均无统计学差异。结论黄芪、南瓜子和槟榔组合对发育中的血吸虫具有较好的抗虫效果,明显优于单用黄芪或南瓜子和槟榔组合的抗虫作用。Objective To observe the difference between single administration of astragalus and combined administration of astragalus,pumpkin seeds and arecas on the treatment of schistosomiasis.Methods 9 groups [infected control group,administration of compound 1～10 d after infection,administration of compound 8～17 d after infection,administration of compound 15～24 d after infection,administration of compound 28～37 d after infection,administration of astragalus 1～10 d after infection,administration of pumpkin seeds 1～10 d after infection,administration of pumpkin seeds and arecas 1～10 d after infection,administration of Praziquantel（PZQ） 36 d after infection at the dose of 300 mg /kg·d] were set up to compare the effect differences of the traditional Chinese medicine compounds at different infective stages.Results The worm reduce rates of the groups administrated compounds 1～10 days,8～ 17 days and 15～24 days after infection were 36.21%,26.74% and 39.04 %（P0.05）,respectively,and the liver egg reduce rates were 58.6%,32.2% and 47.7%（P0.05）,respectively,as compared with control group.The node densities on liver surfaces from treated groups were less than that from control mice.However,as compared the group administrated compounds 28～37 days after infection,administration of astragalus 1～10 d after infection and the group administrated single pumpkin seeds 1 ～10 days after infection as well as the group administrated pumpkin seeds ＋ arecas,there was no difference in worm reduce rate and egg reduce rate when compared with control group.Conclusion The combination of astragalus,pumpkin seeds and arecas could produce a good effect of antischistosomulum,which was better than only use astragalus,or pumpkin seeds or arecas.

关 键 词：日本血吸虫 中药复合剂 黄芪 吡喹酮 杀虫效果 

分 类 号：R285.5[医药卫生—中药学] R383.24[医药卫生—中医学]