胚中段为外植体的花生高效再生体系构建研究  

Regeneration System of Embryo as Explant in Peanut

作　　者：温世杰[1] 刘海燕[1] 梁炫强[1] 

机构地区：[1]广东省农业科学院作物研究所,广东广州510640

出　　处：《花生学报》2010年第2期29-32,共4页Journal of Peanut Science

基　　金：国家高技术研究发展计划(2006AA10Z156);现代农业产业技术体系建设专项资金;粤港关键领域重点突破项目(200849863)

摘　　要：参考幼叶、子叶为外植体诱导丛生芽的方法,利用花生种子胚生长旺盛的特性,以花生种子胚中段为外植体材料建立一个新植株再生体系。结果表明,在含3.0mg/L的6-BA的MS培养基上,培养30d可以诱导出丛生芽,诱导率达到92.5%;丛生芽转至1/2MS+0.2mg/LIBA+0.1mg/LNAA的培养基中培养2～3周,生根形成完整植株。Reference to young leaves, cotyledons as explants induced adventitious shoots, using the peanut seed embryo vigorous growth characteristics, a new regeneration system was established by using the middle piece of embryo of peanut. It laid the foundation for genetic and transgenic manipulation of peanut. The results showed that the adventitious shoots were induced from middle piece of embryo cultured on MS medium supplemented with 3.0mg/L 6-BA for 30 d. Induction frequency of adventitious shoots was 92.5%. Rooting was achieved after adventitious shoot was transplanted to1/2MS＋0.2mg/L IBA＋0.1mg/L NAA medium for 2～3 weeks.

关 键 词：花生 胚中段 再生体系 

分 类 号：S565.2[农业科学—作物学] Q943.1[生物学—植物学]