刃天青显色法检测铜绿假单胞菌对几种抗菌药物的敏感性  

A Resazurin-based Assay for Evaluation of Anti-pseudomonas Aeruginosa Drugs Sensitivity

作　　者：李宙阳[1] 周英[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2010年第3期237-241,共5页Journal of Mountain Agriculture and Biology

基　　金：贵州省中药现代化专项(20075021)

摘　　要：采用刃天青显色法检测3种抗菌药物（头孢哌酮钠、链霉素、青霉素）对铜绿假单胞菌的MIC和最佳作用时间，并比较与微量稀释法的相关性，探讨应用该法的可行性。结果表明：刃天青的试验浓度在75—150ug/mL为宜．细菌接种量1×10 5cfu／孔，肉眼即可判定MIC值结果，判定时间为8h。3种抗菌药物对铜绿假单胞菌的MIC和最佳作用时间分别为：5ug／mL，4～6h；20ug,／mL，6～8h；不敏感。刃天青显色法与稀释法MIC值检测结果一致。本试验结果说明，刃天青显色法具有简便、快速、灵敏度高的特点，能动态地反映细胞随时间变化的生长情况，能检测药物的最佳作用时间。A resazurin-based assay was employed to evaluate MIC and the optimal function time of anti- P. vaeruginosa drugs in the current work. The results demonstrated that under the optimal condition of 75 - 150 ug/mL of resazurin, 1 × 105 cfu/well of bacteria inoculation density, and 8 hours of incubation, the MIC could be judged by naked eye. The MIC for cefoperazone sodium, streptomycin, or penicillin were 5 ug/ml, 20 ug/ml or un-sensitive, respectively. The optimal function times for cefoperazone sodium and streptomycin were 4 -6 h, 6 -8 h, respectively. The results from the resazurin -based assay were consistent with that obtained using conventional dilution assay. Therefore, resazurin assay may dramatically detect the cell growth and evaluate MIC and the optimal function time of anti-bacteria drugs. This resazurin -assay has the considerable advantages to the other assays in both simplicity and convenience.

关 键 词：刃天青 铜绿假单胞菌 MIC 最佳作用时间 

分 类 号：R37[医药卫生—病原生物学]