京尼平固定NGF壳聚糖膜的制备及缓释NGF作用探讨  

Evaluation of the controlled release of NGF from genipin-crosslinked chitosan membranes immobilized with NGF

作　　者：赵微加[1] 魏丽[1] 何江虹[1] 杨宇民[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,江苏226001

出　　处：《交通医学》2010年第3期220-222,共3页Medical Journal of Communications

基　　金：国家自然科学基金资助项目(30970713);国家教育部"新世纪优秀人才"项目(NCET-070466);江苏省自然科学基金项目(BK2009518)

摘　　要：目的:探讨京尼平固定神经生长因子(NGF)壳聚糖膜(GCN膜)缓释NGF的方法和作用。方法:实验组(GCN膜)采用纯天然生物交联剂京尼平将NGF固定在壳聚糖上,采用壳聚糖固定NGF以后的膜与未分化的PC12细胞共培养法观察细胞生长状态。另设阴性对照组(GC膜)不加NGF,阳性对照组(低血清F12K完全培养基中加入50ng/mlNGF)。结果:GCN膜组的PC12细胞在膜中释放出的NGF作用下突起生长明显,与阴性对照组有显著差异。结论:京尼平固定NGF壳聚糖膜可以有效缓释出具有生物活性的NGF,为临床上损伤神经的修复提供新的可能。Objective： To study the effect of the controlled release of nerve growth factor （NGF） from genipin-crosslinked chitosan membranes immobilized with NGF（GCN） which were prepared in our lab on mutagenicity. Methods： The studies were conducted by cultured with the PC12 cells. Results： The neurite outgrowth of PC12 cells which were cultured on GCN membranes showed to be significantly higher than in the control groups. Conclusion： The GCN membranes can be materials for sustained release of bioactive NGF and provide a future possibility for therapeutic nerve repair.

关 键 词：壳聚糖 京尼平 神经生长因子 缓释 PC12细胞 

分 类 号：R318[医药卫生—生物医学工程]