豌豆PsSGR基因cDNA的分离及其遗传功能分析  

Isolation of PsSGR cDNA in Pisum sativum and Genetic Analysis of its Function in Arabidopsis

作　　者：周茜[1] 任国栋[1] 周霄[1] 蒯本科[1] 

机构地区：[1]复旦大学生命科学学院生物化学系,上海200433

出　　处：《复旦学报（自然科学版）》2010年第3期309-314,322,F0003,共8页Journal of Fudan University：Natural Science

摘　　要：拟南芥滞绿基因AtNYE1是衰老过程中调控叶绿素降解的关键基因.利用cDNA末端快速扩增(RACE)技术,在豌豆(Pisum sativum)中分离获得了黄色子叶豌豆PsSGR基因和突变体绿色子叶豌豆PssgrGr基因的全长cDNA.为了验证AtNYE1同源基因PsSGR的功能,将黄色子叶豌豆PsSGR和绿色子叶豌豆PssgrЛ2775的编码区序列分别构建到带有花椰菜花叶病毒35S组成型强启动子和拟南芥叶片衰老特异诱导型启动子SAG12的植物表达载体上,经农杆菌介导、通过花序浸染法转化拟南芥滞绿突变体nye1-1.互补实验结果显示,PsSGR基因能够恢复拟南芥滞绿突变体nye1-1的滞绿表型,而PssgrЛ2775基因则不能.这一结果表明,豌豆PsSGR是负责豌豆子叶中叶绿素降解的调控因子.A stay-green gene AtNYE1 was previously identified in Arabidopsis thaliana,playing a key role in the regulation of Chlorophyll degradation during senescence.The NYE1 homolog in Pisum sativum was isolated via rapid amplification of cDNA ends（RACE） technology.The PsSGR/Pssgr^JI2775 gene,identified from the green cotyledon variety and driven by CaMV35S promoter or SAG12 promoter,respectively,was subsequently introduced into nye1-1 via the floral-dipping method.The results demonstrated that PsSGR,but not Pssgr^JI2775,could rescue the stay-green phenotype of nye1-1,suggesting that PsSGR is a true ortholog of AtNYE1.

关 键 词：叶绿素降解 滞绿 豌豆 NYE1 CDNA末端快速扩增技术 功能互补 

分 类 号：Q94[生物学—植物学]