联合培养VECs与ADSCs与部分脱蛋白生物骨体外构建组织工程骨的实验研究  

Experimental Study on Construction of Tissue Engineering Bone with the Co-culture System Composed of Adipose-Derived Stromal Cells and Vascular Endothelial Cells and Partially Deproteinised Biologic Bone In Vitro

作　　者：刘流[1] 王福科[1] 赵德萍[1] 何晓光[1] 钟玲[1] 代晓明[1] 李逸松[1] 

机构地区：[1]昆明医学院第一附属医院

出　　处：《中国生物美容》2010年第1期1-9,共9页China Bio-beauty

基　　金：国家自然科学基金(30960388);云南科技厅联合专项基金资助项目(2007C0007R);昆明医学院博士创新基金项目(KM2007D03)

摘　　要：目的本实验旨在研究体外血管内皮细胞和脂肪来源的联合培养体系在部分脱蛋白生物骨支架材料上的黏附、增殖,以及二者体外培养构建组织工程骨时移植入体内的最佳时机,为体内构建组织工程骨提供理论依据。方法取血管内皮细胞、脂肪干细胞和1:1联合培养细胞分别接种部分脱蛋白生物骨片,MTT法检测吸光度,评价三种不同细胞组在PDPBB生长情况,分析联合培养细胞在支架材料上增殖情况,扫描电镜观察不同细胞组在PDPBB生长情况。结果各细胞组在PDPBB上吸光度逐渐增加,第10天均达到高峰,1:1混合细胞组最高,各细胞组之间差异有统计学意义(P<0.01);10天可见联合培养细胞细胞组大量细胞与PDPBB附着,呈巢状分布。结论 1:1混合细胞在PDPBB支架材料上的增殖优于单种细胞,10天为最佳移植时机。Objective To explore the adhesion and proliferation of the co-culture system composed of ADSCs and VECs on partially deproteinised biologic bone（PDPBB） in vitro and the most proper occasion to transplant of construting tissue engineering bone with the co-culture system and partially deproteinised biologic bone, to provide theoretical basis for construting tissue engineering bone in vivo. Methods The PDPBB materials were inoculated separately with VECs, ADSCs and the co-culture cells according to the ratio of 1：1 ,The absorbance was tested by MTT automated for assessment the adhesive and proliferation conditions of three different cell on PDPBB, and it was observed with a scanning electron microscope. Result The absorbance of every group increased gradually and it reach tip on tenth day, the highest was the co-culture cells group according to the ratio of 1 ：l,it have significantly different when every group was compared each other（P〈0.01）; On 10th day, a great deal of cells adhere on the PDPBB materials in the co-culture cells group and showed a nestlike distribution. Conclusion The adhesive and proliferation of the co-culture cells was better than the single kind cells and the most proper occasion to transplanted is the tenth day.

关 键 词：部分脱蛋白生物骨 纤维粘连蛋白 血管内皮细胞 脂肪干细胞 联合培养 

分 类 号：R318.08[医药卫生—生物医学工程]