由小麦×玉米获得的普通小麦加倍单倍体后代的RFLP变异  

RFLP Variations of Common Wheat Doubled Haploid Progenies from Wheat × Maize Crosses

作　　者：陈纯贤[1] 孙敬三[2] 朱立煌[1] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]中国科学院植物研究所,北京100093

出　　处：《Acta Botanica Sinica》1999年第1期55-59,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：用小麦（Ｔｒｉｔｉｃｕｍ　ａｅｓｔｉｖｕｍＬ．）的ｒＤＮＡ克隆ｐＴａ７１和与小麦基因组有部分同源性的玉米（ＺｅａｍａｙｓＬ．）ＤＮＡ克隆作探针，对由小麦ｘ玉米获得的普通小麦加倍单倍体（ＤＨ）后代群体进行ＲＦＬＰ分析。结果发现，不但用ｐＴａ７１在这些ＤＨ后代中检测到ｒＤＮＡ所发生的明显的减少和扩增及非转录间隔区的限制性片段长度的变化，而且用与小麦基因组部分同源的玉米克隆ＭＲ１３和ＭＲ５０在一些ＤＨ后代中检测到缺失变异，特别是用ＭＲ１３在普通小麦ＤＨ系的１８号株的基因组中检测到大幅度的限制性片段长度的变化，即原来的４．３ｋｂ的强信号带消失，取而代之的是４０．０ｋｂ、２．５ｋｂ和２．０ｋｂ三条杂交带。这可能与小麦基因组ＤＮＡ较大的重排事件有关，也可能是由外源的玉米ＤＮＡ插入造成的。Wheat (Triticum aestivum L. ) and maize (Zea mays L. ) crosses (the chromosome eliminationsystem) can be used to produce frequently a large number of doubled haploid (DH) wheat lines by embryo res-cue and doubling treatment. The resulting DH lines are genetically homogeneous. Significant RFLP variationswere detected in common wheat DH progenies from wheat and maize crosses by using wheat rDNA clone pTa71and two maize DNA clones (MR13 and MB50) homologous to wheat genome as probes. The results revealedthat the copy number and restriction fragment length of rDNA in some wheat DH progenies was changed, andalso that deletion was detected in several DH plants when probed with MR13 and MR50. In particular, theRFLP pattern of DH line No. 18 was changed using MR13 as a probe. In this line, be new bands,40.0 kb, 2.5 kb and 2.0 kb emerged while a 4.3 kb intense band from the parental common wheat genomedisappearedd. This change may be related to a quite large DNA rearrangement within the wheat genomic DNA oran insertion by alien maize DNA fragment.

关 键 词：小麦 玉米 远缘杂交 加倍单倍体 RFLP 

分 类 号：S512.103.5[农业科学—作物学] S513.035.1