DBD等离子体针杀灭口腔混合菌的实验研究  

Investigation of DBD Plasma Needle Applications in Oral Mixed Bacteria

作　　者：于航[1,2] 冯玉玲[1] 冯克成[1] 黄骏[1] 吕国华[2] 杨思泽[2] 

机构地区：[1]长春理工大学理学院,长春130022 [2]中国科学院北京物理研究所,北京100080

出　　处：《长春理工大学学报（自然科学版）》2010年第2期99-102,106,共5页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：国家自然科学基金重点项目(10735090);北京市科技计划项目(Z090605006009022)

摘　　要：利用介质阻挡放电dielectric barrier discharge(DBD)装置产生的针状等离子体,对口腔混合菌的杀灭以及对成纤维细胞的作用进行了研究。通过调节装置内电极氧气的流量来控制装置输出等离子体的含量,从而使其产生的能够与细菌结合的氧自由基含量改变。经过研究发现,适当的增加氧气流量可以增加氧自由基的含量,当增加的氧气流量超过一定的数值,会导致氧自由基的数量减少,而当氧气流量在25ml·min1时氧自由基含量较多,杀菌效果较好。为了模拟实际口腔环境,从小白鼠体内提取出成纤维细胞,通过成纤维细胞每毫升群落个数和电压之间的关系曲线,发现在相同的电压下,氧气流量越大,细胞存活数量越少,而在相同的氧气流量下,电压越大,细胞的存活数量越少。最后给出合适的实验参数条件:在标准大气压条件下,处理电压为15(X170)V,氧气流量为25ml·min1,氦气流量为0.1m3·h1,处理时间为20s,这样即能够达到较好的杀菌效果,又对人体有用细胞的伤害较小。This article studied dielectric barrier discharge plasma needle on the killing of oral mixed bacteria and its effect on fibroblasts.By adjusting the flow of inner electrode oxygen to control plasma levels of output device,so that changes the content of oxygen free radicals in order to achieve better sterilizing effect.The research indicates that an appropriate increase in oxygen flow rate can increase the content of oxygen free radicals,the increasing of oxygen flow rate exceeds a certain value will lead to reduction in the number of oxygen free radicals,but when the oxygen flow at 25ml · min 1 contains more oxygen free radicals and sterilization was better.Body extracted from mouse fibroblast simulate the actual oral environment,by the curve between fibroblasts CFU · ml 1 and voltage found under the same voltage,the greater the oxygen flow rate the less the number of cell survival,while in the same oxygen flow rate,the greater the voltage the less the number of cell survival.Finally,the ideal experimental parameters are in the atmospheric conditions,output voltage of 15（X170）V,oxygen flow rate of 25ml · min 1,helium flow rate of 0.1 m3 · h 1,the processing time of 20 seconds,in such conditions can achieve better sterilization effect and less detrimental to the human body.

关 键 词：等离子体 介质阻挡放电 口腔混合菌 成纤维细胞 

分 类 号：N33[自然科学总论]