草莓CO基因克隆与表达分析  

Cloning and expression analysis of CONSTANS gene in strawberry

作　　者：赵志琴[1] 安丽君[1] 李芳[1] 李天红[1] 

机构地区：[1]中国农业大学农学与生物技术学院北京市果树逆境生理与分子生物学重点开放实验室,北京100193

出　　处：《中国农业大学学报》2010年第1期19-24,共6页Journal of China Agricultural University

基　　金：新世纪人才支持计划(NCET-06-0108);国家"十一五"科技支撑计划(2007BAD36B02);国家自然科学基金资助项目(30871696)

摘　　要：采用RT-PCR和RACE技术从短日照草莓品种‘卡姆罗莎’(Fragaria×ananassaDuch.cv.‘Cama-rosa’)嫩叶中克隆得到与草莓成花相关的基因,命名为FaCO(GenBank登录号,FJ377616)。该基因cDNA全长1475 bp,1 152 bp的开放阅读框编码一个含有384个氨基酸,分子质量为42 ku的蛋白。该蛋白具有典型的CO同源蛋白结构,包含B-box1,B-box2,CCT结构域及COOH区域。序列分析结果表明,草莓CO蛋白与其他物种的CO蛋白具有较高的同源性,其中与杨树(Populus deltoids)的CO蛋白同源性最高,差异为80%。构建pGEX-4T-1/FaCO原核表达体系,获得了分子质量为60 ku的融合蛋白。A CONSTANS homologous gene named FaCO was cloned from young leaves of strawberry（Fragaria ×ananassa Duch.cv.＇Camarosa＇） by RT-PCR and RACE technology.The gene had a cDNA sequence with 1 475 bp in length and contained an open reading frame of 1 152 bp which coding a deduced 384 amino acids protein.The peptide had two B-boxes,a CCT domain and a COOH region shared highly homology with CO of other plants,especially with CO of Populus deltoids,to 80%.The pGEX-4T-1/FaCO prokaryotic expression systems were constructed and induced.SDS-PAGE analysis showed that the rFaCO molecular weight was about 60 ku.

关 键 词：CONSTANS基因 草莓 克隆 表达 

分 类 号：S668.4[农业科学—果树学]